The first echinoderm gamma-interferon-inducible lysosomal thiol reductase (GILT) identified from sea cucumber (Stichopus monotuberculatus)

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	The first echinoderm gamma-interferon-inducible lysosomal thiol reductase (GILT) identified from sea cucumber (Stichopus monotuberculatus)
	Ren, Chunhua 1; Chen, Ting 1; Jiang, Xiao 1; Luo, Xing 1; Wang, Yanhong 1; Hu, Chaoqun 1
刊名	FISH & SHELLFISH IMMUNOLOGY
	2015
卷号	42 期号:1 页码:41-49
关键词	Gamma-interferon-inducible lysosomal thiol reductase Stichopus monotuberculatus Gene structure Thiol reductase activity Innate immune
通讯作者	rosemary166@sina.com ; chan1010@scsio.ac.cn ; jiangxiao4367@126.com ; luoxingzky@163.com ; yhwang@scsio.ac.cn ; hucq@scsio.ac.cn
英文摘要	Gamma-interferon-inducible lysosomal thiol reductase (GILT) has been described as a key enzyme that facilitating the processing and presentation of major histocompatibility complex class II-restricted antigen in mammals. In this study, the first echinoderm GILT named StmGILT was identified from sea cucumber (Stichopus monotuberculatus). The StmGILT cDNA is 1529 bp in length, containing a 5'-un-translated region (UTR) of 87 bp, a 3'-UTR of 674 bp and an open reading frame (ORF) of 768 bp that encoding a protein of 255 amino acids with a deduced molecular weight of 27.82 kDa and a predicted isoelectric point of 4.73. The putative StmGILT protein possesses all the main characteristics of known GILT proteins, including a signature sequence, a reductase active site CXXC, twelve conserved cysteines, and two potential N-linked glycosylation sites. For the gene structure, StmGILT contains four exons separated by three introns. In the promoter region of StmGILT gene, an NF-kappa B binding site and an IFN-gamma activation site were found. The thiol reductase activity of recombinant StmGILT protein was also demonstrated in this study. In addition, the highest level of mRNA expression was noticed in coelomocytes of S. monotuberculatus. In in vitro experiments performed in coelomocytes, the expression of StmGILT mRNA was significantly up-regulated by lipopolysaccharides (LPS), inactivated bacteria or polyriboinosinic polyribocytidylic acid [poly (LC)] challenge, suggested that the sea cucumber GILT might play critical roles in the innate immune defending against bacterial and viral infections. (C) 2014 Elsevier Ltd. All rights reserved.
学科主题	Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences
原文出处	1050-4648
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/14897]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
作者单位	1.[Ren, Chunhua 2.Chen, Ting 3.Jiang, Xiao 4.Luo, Xing 5.Wang, Yanhong 6.Hu, Chaoqun] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Appl Marine Biol Guangdong Prov, CAS Key Lab Trop Marine Bioresources & Ecol LMB, Guangzhou 510301, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Ren, Chunhua,Chen, Ting,Jiang, Xiao,et al. The first echinoderm gamma-interferon-inducible lysosomal thiol reductase (GILT) identified from sea cucumber (Stichopus monotuberculatus)[J]. FISH & SHELLFISH IMMUNOLOGY,2015,42(1):41-49.
APA	Ren, Chunhua,Chen, Ting,Jiang, Xiao,Luo, Xing,Wang, Yanhong,&Hu, Chaoqun.(2015).The first echinoderm gamma-interferon-inducible lysosomal thiol reductase (GILT) identified from sea cucumber (Stichopus monotuberculatus).FISH & SHELLFISH IMMUNOLOGY,42(1),41-49.
MLA	Ren, Chunhua,et al."The first echinoderm gamma-interferon-inducible lysosomal thiol reductase (GILT) identified from sea cucumber (Stichopus monotuberculatus)".FISH & SHELLFISH IMMUNOLOGY 42.1(2015):41-49.