Ultrafast Tracking of a Single Live Virion During the Invagination of a Cell Membrane

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	Ultrafast Tracking of a Single Live Virion During the Invagination of a Cell Membrane
	Pan, Yangang 1; Wang, Shaowen 1; Shan, Yuping 1; Zhang, Dinglin 1; Gao, Jing 1; Zhang, Min 1; Liu, Shuheng 1; Cai, Mingjun 1; Xu, Haijiao 1; Li, Guohui 1
刊名	SMALL
	2015
卷号	11 期号:23 页码:2782-2788
通讯作者	ghli@dicp.ac.cn ; qinqw@scsio.ac.cn ; hdwang@ciac.ac.cn
英文摘要	The first step in most viral infections is the penetration of the cell membrane via endocytosis. However, the underlying mechanism of this important process has not been quantitatively characterized; for example, the velocity and force of a single virion during invagination remain unknown. Here, the endocytosis of a single live virion (Singapore grouper iridovirus, SGIV) through the apical membranes of a host cell is monitored by developing and using a novel ultrafast (at the microsecond level) tracking technique: force tracing. For the first time, these results unambiguously reveal that the maximum velocity during the cell entry of a single SGIV by membrane invagination is approximately 200 nm s(-1), the endocytic force is approximately 60.8 +/- 18.5 pN, and the binding energy density increases with the engulfment depth. This report utilizing high temporospatial resolution (subnanometer and microsecond levels) approaches provides new insight into the dynamic process of viral infection via endocytosis and the mechanism of membrane invagination at the single-particle level.
学科主题	Chemistry; Science & Technology - Other Topics; Materials Science; Physics
原文出处	1613-6810
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/14813]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
作者单位	1.[Pan, Yangang 2.Shan, Yuping 3.Gao, Jing 4.Zhang, Min 5.Liu, Shuheng 6.Cai, Mingjun 7.Xu, Haijiao 8.Wang, Hongda] Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Jilin, Peoples R China 9.[Pan, Yangang 10.Wang, Shaowen
推荐引用方式 GB/T 7714	Pan, Yangang,Wang, Shaowen,Shan, Yuping,et al. Ultrafast Tracking of a Single Live Virion During the Invagination of a Cell Membrane[J]. SMALL,2015,11(23):2782-2788.
APA	Pan, Yangang.,Wang, Shaowen.,Shan, Yuping.,Zhang, Dinglin.,Gao, Jing.,...&Wang, Hongda.(2015).Ultrafast Tracking of a Single Live Virion During the Invagination of a Cell Membrane.SMALL,11(23),2782-2788.
MLA	Pan, Yangang,et al."Ultrafast Tracking of a Single Live Virion During the Invagination of a Cell Membrane".SMALL 11.23(2015):2782-2788.