QQ客服 官方微博 反馈留言

题名	生物芯片扫描仪的关键技术研究
作者	王文奎
学位类别	博士
答辩日期	2002
授予单位	中国科学院上海光学精密机械研究所
导师	胡企铨
关键词	生物芯片 扫描仪 线阵列CCD 窄带干涉滤光片 荧光染料
其他题名	Study of Biochip Scanner Key Technology
中文摘要	本论文工作是结合中国科学院知识创新项目："基因芯片检测关键技术研究"进行的。本文研究了一种新型的生物芯片扫描仪。在该扫描仪中采用了高灵敏度的线阵列CCD作探测器，扫描机构采用一维移动平台。线阵列CCD作探测器比光电倍增管PMT作探测器对激光器的噪声要求低。我们采用线形发生器使得激光光源变成很细很窄的一条能量分布均匀的激光束激发生物芯片，用532nm和650nm波长激光作为激发光源激发标记在生物芯片上的荧光染料cy3TM和cy5TM以产生荧光信号。为了提高扫描仪的信噪比，本扫描仪中采用了两种窄带干涉滤光片，分别用来滤除激发光源光束。由于采用了合理的成像系统，通过选择成像镜头的位置可以实现不同的分辨率。实验表明，本扫描仪的扫描精度可达14微米，也可以达到5或10微米的分辨率。本扫描仪扫描一片标准载玻片（23mm*73mm）仅需不到5分钟的时间。本文还介绍了自行开发的软件，通过该软件控制本扫描仪扫描生物芯片样品得到了理想的图像。用该软件既可以输出纯文本的数据文件供第三方软件分析用，可以输出256色位图文件供我们直观分析用，还可以选择CCD积分时间、采样频率、成像范围，及扫描速度。实验结果表明该扫描仪可以用于生物基因芯片的扫描检测。
英文摘要	This work is supported by Knowledge Innovation Programme of Chinese Academy of Sciences "Study of Gene Chip Detection Key Technology". In this thesis, a novel gene chip scanner is studied. In this scanner, a high sensitive linear array CCD is used as detector and one direction movable platform is chosen to drive the biochip. This scanner doesn't need the laser to have very low noise which is critical to the scanner whose detector is PMT. A laser line generator is used to generate a very thin and uniform intensity laser line to excite the fluorescence. 532nm and 650nm lasers are chosen to excite the biochip labeled with Cy3? and Cy5? fluorescence dyes to emit the fluorescence signal. In order to increase the ratio of signal to noise, two narrow band interference filters are used in this scanner to filter the laser beam which functions as noise in the fluorescence signal detection. A different resolution can be achieved by selecting the position of the objective lens due to the reasonable objective optics system. The experiment shows that a resolution of 14 microns can be achieved. 5 microns or 10 microns resolution can be achieved, too. It takes the scanner less than 5 minutes to scan a standard slide (23mm X 75mm). In this thesis, software developed by us to control the scanner is introduced. By running the software, the scanner has succeeded in scanning a standard slide with fluorescence dye dotted on and an ideal image is acquired. With the software, we can get text file to be analyzed by our partner, a standard 256 colors windows bitmap can be achieved to be analyzed by us. The integral time of CCD, the signal collection frequency, the scanning area and the scanning speed can be selected, too. The experiment shows that this scanner can be used to detect the biochip.
语种	中文
内容类型	学位论文
源URL	[http://ir.siom.ac.cn/handle/181231/15399]
专题	上海光学精密机械研究所_学位论文
推荐引用方式 GB/T 7714	王文奎. 生物芯片扫描仪的关键技术研究[D]. 中国科学院上海光学精密机械研究所. 2002.

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。