Nonspecific Organelle-Targeting Strategy with Core-Shell Nanoparticles of Varied Lipid Components/Ratios

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	Nonspecific Organelle-Targeting Strategy with Core-Shell Nanoparticles of Varied Lipid Components/Ratios
	Zhang L; Sun JS; Wang YL; Wang JC; Shi XH; Hu GQ(胡国庆); Hu GQ(胡国庆)
刊名	ANALYTICAL CHEMISTRY
	2017
通讯作者邮箱	sunjs@nanoctr.cn
卷号	88 期号:14 页码:7344-7351
ISSN号	0003-2700
通讯作者	Sun, JS (reprint author), Natl Ctr Nanosci & Technol, CAS Ctr Excellence Nanosci, CAS Key Lab Biol Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China.
产权排序	[Zhang, Lu] Capital Normal Univ, Dept Chem, Beijing 100048, Peoples R China; [Sun, Jiashu; Shi, Xinghua] Natl Ctr Nanosci & Technol, CAS Ctr Excellence Nanosci, CAS Key Lab Biol Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China; [Wang, Yilian; Wang, Jiancheng] Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China; [Hu, Guoqing] Chinese Acad Sci, Inst Mech, State Key Lab Nonlinear Mech, Beijing 100190, Peoples R China
中文摘要	We report a nonspecific organelle-targeting strategy through one-step microfluidic fabrication and screening of a library of surface charge- and lipid components/ratios-varied lipid shell polymer core nanoparticles. Different from the common strategy relying on the use of organelle-targeted moieties conjugated onto the surface of nanoparticles, here, we program the distribution of hybrid nanoparticles in lysosomes or mitochondria by tuning the lipid components/ratios in shell. Hybrid nanoparticles with 60% 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) and 20% 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) can intracellularly target mitochondria in both in vitro and in vivo models. While replacing DOPE with the same amount of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), the nanoparticles do not show mitochondrial targeting, indicating an incremental effect of cationic and fusogenic lipids on lysosomal escape which is further studied by molecular dynamics simulations. This work unveils the lipid-regulated subcellular distribution of hybrid nanoparticles in which target moieties and complex synthetic steps are avoided.
分类号	一类
类目[WOS]	Chemistry, Analytical
研究领域[WOS]	Chemistry
收录类别	SCI ; EI
原文出处	http://dx.doi.org/10.1021/acs.analchem.6b01749
语种	英语
WOS记录号	WOS:000380296800054
内容类型	期刊论文
源URL	[http://dspace.imech.ac.cn/handle/311007/59761]
专题	力学研究所_非线性力学国家重点实验室
推荐引用方式 GB/T 7714	Zhang L,Sun JS,Wang YL,et al. Nonspecific Organelle-Targeting Strategy with Core-Shell Nanoparticles of Varied Lipid Components/Ratios[J]. ANALYTICAL CHEMISTRY,2017,88(14):7344-7351.
APA	Zhang L.,Sun JS.,Wang YL.,Wang JC.,Shi XH.,...&Hu GQ.(2017).Nonspecific Organelle-Targeting Strategy with Core-Shell Nanoparticles of Varied Lipid Components/Ratios.ANALYTICAL CHEMISTRY,88(14),7344-7351.
MLA	Zhang L,et al."Nonspecific Organelle-Targeting Strategy with Core-Shell Nanoparticles of Varied Lipid Components/Ratios".ANALYTICAL CHEMISTRY 88.14(2017):7344-7351.