Asymmetrical flow field-flow fractionation coupled with multi-angle laser light scattering for stability comparison of virus-like particles in different solution environments

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	Asymmetrical flow field-flow fractionation coupled with multi-angle laser light scattering for stability comparison of virus-like particles in different solution environments
	Chen, Yi 1,2; Zhang, Yan 1; Zhou, Yuefang 1; Luo, Jian 1; Su, Zhiguo 1,3
刊名	VACCINE
	2016-06-08
卷号	34 期号:27 页码:3164-3170
关键词	Asymmetrical flow field-flow fractionation Virus-like particle Antigenicity Hepatitis B surface antigen Aggregation
ISSN号	0264-410X
英文摘要	The stabilities of two commercially available virus like particles, CHO-HBsAg expressed by Chinese hamster ovary (CHO) cells and Hans-HBsAg expressed by Hansenula polymorpha (Hans), were compared using AF4-MALLS under different treatment processes. The initial molecular weight and hydrodynamic diameter of CHO-HBsAg measured with AF4-MALLS were 4727 kDa and 29.4nm, while those of Hans-HBsAg were 3039 kDa and 22.8 nm respectively. In salt solution of 2 M ammonium sulfate, the molecular weight and size of CHO-HBsAg had little change, and its antigenicity remained 95%, while those of Hans-HBsAg changed greatly, resulting in aggregation and 75% antigenicity loss. In freeze-thaw operations, Hans-HBsAg aggregated heavily. Most of the aggregates precipitated and the rest soluble aggregates reached 10(5)-10(6) kDa in molecular weight. The antigenicity of Hatis-HBsAg decreased to 26.9% after five freeze-thaw cycles. For CHO-HBsAg, there was no obvious aggregation in freeze-thaw, and the antigenicity retained above 98%. In heating process, Hans-HBsAg gradually aggregated to large particles with temperature and the antigenicity decreased to 10% when the temperature reached 80 degrees C. In contrast, CHO-HBsAg would not aggregate with temperature, remained 92% antigenicity at 80 degrees C. The study demonstrated that CHO-HBsAg appeared to be a superior vaccine antigen in term of particle stability and constant antigenicity, which are important in production, transportation and storage. (C) 2016 Elsevier Ltd. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Immunology ; Medicine, Research & Experimental
研究领域[WOS]	Immunology ; Research & Experimental Medicine
关键词[WOS]	B SURFACE-ANTIGEN ; VACCINE FORMULATION ; SIZE ; IMMUNOGENICITY ; STABILIZATION ; AGGREGATION ; YEAST ; CELLS
收录类别	SCI
语种	英语
WOS记录号	WOS:000378667900023
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/21172]
专题	过程工程研究所_生化工程国家重点实验室
作者单位	1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beiertiao 1, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Jiangsu Natl Synerget Innovat Ctr Adv Mat SICAM, Nanjing 210023, Jiangsu, Peoples R China
推荐引用方式 GB/T 7714	Chen, Yi,Zhang, Yan,Zhou, Yuefang,et al. Asymmetrical flow field-flow fractionation coupled with multi-angle laser light scattering for stability comparison of virus-like particles in different solution environments[J]. VACCINE,2016,34(27):3164-3170.
APA	Chen, Yi,Zhang, Yan,Zhou, Yuefang,Luo, Jian,&Su, Zhiguo.(2016).Asymmetrical flow field-flow fractionation coupled with multi-angle laser light scattering for stability comparison of virus-like particles in different solution environments.VACCINE,34(27),3164-3170.
MLA	Chen, Yi,et al."Asymmetrical flow field-flow fractionation coupled with multi-angle laser light scattering for stability comparison of virus-like particles in different solution environments".VACCINE 34.27(2016):3164-3170.