CRASP: CFP reconstitution across synaptic partners

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	CRASP: CFP reconstitution across synaptic partners
	Li, YM; Guo, AK; Li, H
刊名	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
	2016
卷号	469 期号:3 页码:352-356
关键词	GREEN FLUORESCENT PROTEIN MUSHROOM BODY DROSOPHILA CIRCUIT REPRESENTATION NEURONS GRASP MAP
通讯作者	Li, H (reprint author), Chinese Acad Sci, Inst Neurosci, State Key Lab Neurosci, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China.,hli@ion.ac.cn
英文摘要	Mapping the pattern of connectivity between neurons is widely regarded to be critical for understanding the nervous system. GRASP (GFP reconstitution across synaptic partners) has been used as a promising method for mapping neuronal connectivity, but is currently available in the green color only, limiting its potential applications. Here we demonstrate CRASP (CFP reconstitution across synaptic partners), a cyan colored version of GRASP. We validated the system in HEK 293T cells, and generated transgenic Drosophila lines to show that the system could reliably detect neuronal contacts in the brain. Furthermore, we showed that the CRASP signal could be selectively amplified using standard immunohistochemistry methods. The CRASP system adds to the toolkit available to researchers for mapping neuronal connectivity, and substantially expands the potential application of GRASP-like strategies. (C) 2015 Elsevier Inc. All rights reserved.
学科主题	Biochemistry & Molecular Biology; Biophysics
WOS标题词	Biochemistry & Molecular Biology ; Biophysics
语种	英语
WOS记录号	WOS:000369352800004
内容类型	期刊论文
源URL	[http://ir.sibs.ac.cn/handle/331001/4045]
专题	上海神经科学研究所_神经所(总)
推荐引用方式 GB/T 7714	Li, YM,Guo, AK,Li, H. CRASP: CFP reconstitution across synaptic partners[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2016,469(3):352-356.
APA	Li, YM,Guo, AK,&Li, H.(2016).CRASP: CFP reconstitution across synaptic partners.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,469(3),352-356.
MLA	Li, YM,et al."CRASP: CFP reconstitution across synaptic partners".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 469.3(2016):352-356.