Nitidine chloride-assisted bio-functionalization of reduced graphene oxide by bovine serum albumin for impedimetric immunosensing

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	Nitidine chloride-assisted bio-functionalization of reduced graphene oxide by bovine serum albumin for impedimetric immunosensing
	Li, Y; Zhang, Z; Zhang, YT; Deng, DM; Luo, LQ; Han, BS; Fan, CH
刊名	BIOSENSORS & BIOELECTRONICS
	2016
卷号	79 期号:-页码:536—542
关键词	CARBON NANOTUBES CARCINOEMBRYONIC ANTIGEN IMPEDANCE SPECTROSCOPY GOLD NANOPARTICLES BIOMARKER DETECTION SIGNALING PATHWAY BINDING CANCER NANOCOMPOSITE IMMUNOASSAY
ISSN号	0956-5663
通讯作者	Luo, LQ (reprint author), Shanghai Univ, Coll Sci, Shanghai 200444, Peoples R China. ; Han, BS (reprint author), Shanghai Jiao Tong Univ, Shanghai Xinhua Hosp, Dept Surg, Shanghai 200092, Peoples R China.
英文摘要	A novel protocol of label-free electrochemical impedance immunosensor based on bovine serum albumin-nitidine chloride-reduced graphene oxide (BSA-NC-rGO) nanocomposite was proposed for quantitative determination of carcino-embryonic antigen (CEA). BSA was anchored to rGO via the aromatic plane of NC by pi-stacking interaction to realize bio-functionalization of rGO, and then gold nanoparticles (AuNPs) were electrodeposited onto the surface of BSA-NC-rGO nanocomposite. The morphology, conductivity and interaction of different nanocomposites were characterized by scanning electron microscopy, cyclic voltammetry, electrochemical impedance spectroscopy (EIS) and UV-vis spectrum. CEA monoclonal antibody (anti-CEA) was conjugated to AuNPs via gold-thiol chemistry to construct electrochemical immunosensing platform, and the specific immunoreaction between CEA and anti-CEA was monitored by EIS. Under optimum conditions, CEA could be quantified in a wide range of 0.1-200 ng mL(-1) (R=0.9948) with low detection limit of 0.067 ng mL(-1). The proposed immunosensor exhibited great potential for detecting blood samples. (C) 2015 Elsevier B.V. All rights reserved.
收录类别	SCI
语种	英语
WOS记录号	WOS:000370309200075
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/25786]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
推荐引用方式 GB/T 7714	Li, Y,Zhang, Z,Zhang, YT,et al. Nitidine chloride-assisted bio-functionalization of reduced graphene oxide by bovine serum albumin for impedimetric immunosensing[J]. BIOSENSORS & BIOELECTRONICS,2016,79(-):536—542.
APA	Li, Y.,Zhang, Z.,Zhang, YT.,Deng, DM.,Luo, LQ.,...&Fan, CH.(2016).Nitidine chloride-assisted bio-functionalization of reduced graphene oxide by bovine serum albumin for impedimetric immunosensing.BIOSENSORS & BIOELECTRONICS,79(-),536—542.
MLA	Li, Y,et al."Nitidine chloride-assisted bio-functionalization of reduced graphene oxide by bovine serum albumin for impedimetric immunosensing".BIOSENSORS & BIOELECTRONICS 79.-(2016):536—542.