Functional Analysis of Two L-Arabinose Transporters from Filamentous Fungi Reveals Promising Characteristics for Improved Pentose Utilization in Saccharomyces cerevisiae

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	Functional Analysis of Two L-Arabinose Transporters from Filamentous Fungi Reveals Promising Characteristics for Improved Pentose Utilization in Saccharomyces cerevisiae
	Li, Jingen 1; Xu, Jing 1,3; Cai, Pengli 1; Wang, Bang 1; Ma, Yanhe 1; Benz, J. Philipp 2; Tian, Chaoguang 1
刊名	APPLIED AND ENVIRONMENTAL MICROBIOLOGY
	2015-06-01
卷号	81 期号:12 页码:4062-4070
英文摘要	Limited uptake is one of the bottlenecks for L-arabinose fermentation from lignocellulosic hydrolysates in engineered Saccharomyces cerevisiae. This study characterized two novel L-arabinose transporters, LAT-1 from Neurospora crassa and MtLAT-1 from Myceliophthora thermophila. Although the two proteins share high identity (about 83%), they display different substrate specificities. Sugar transport assays using the S. cerevisiae strain EBY. VW4000 indicated that LAT-1 accepts a broad substrate spectrum. In contrast, MtLAT-1 appeared much more specific for L-arabinose. Determination of the kinetic properties of both transporters revealed that the Km values of LAT-1 and MtLAT-1 for L-arabinose were 58.12 +/- 4.06 mM and 29.39 +/- 3.60 mM, respectively, with corresponding V-max values of 116.7 +/- 3.0 mmol/h/g dry cell weight (DCW) and 10.29 +/- 0.35 mmol/h/g DCW, respectively. In addition, both transporters were found to use a proton-coupled symport mechanism and showed only partial inhibition by D-glucose during L-arabinose uptake. Moreover, LAT-1 and MtLAT-1 were expressed in the S. cerevisiae strain BSW2AP containing an L-arabinose metabolic pathway. Both recombinant strains exhibited much faster L-arabinose utilization, greater biomass accumulation, and higher ethanol production than the control strain. In conclusion, because of higher maximum velocities and reduced inhibition by D-glucose, the genes for the two characterized transporters are promising targets for improved L-arabinose utilization and fermentation in S. cerevisiae.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology ; Microbiology
研究领域[WOS]	Biotechnology & Applied Microbiology ; Microbiology
关键词[WOS]	YEAST HEXOSE TRANSPORTERS ; ALCOHOLIC FERMENTATION ; XYLOSE UTILIZATION ; NEUROSPORA-CRASSA ; CANDIDA-INTERMEDIA ; SYSTEMS-ANALYSIS ; EXPRESSION ; ETHANOL ; PERFORMANCE ; CELLOBIOSE
收录类别	SCI
语种	英语
WOS记录号	WOS:000354864000016
内容类型	期刊论文
源URL	[http://124.16.173.210/handle/834782/1479]
专题	天津工业生物技术研究所_微生物功能基因组学与生物技术实验室田朝光_期刊论文
作者单位	1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Key Lab Syst Microbial Biotechnol, Tianjin, Peoples R China 2.Tech Univ Munich, Holzforschung Munchen, TUM Sch Life Sci Weihenstephan, Freising Weihenstephan, Germany 3.Hubei Univ, Coll Life Sci, Wuhan, Peoples R China
推荐引用方式 GB/T 7714	Li, Jingen,Xu, Jing,Cai, Pengli,et al. Functional Analysis of Two L-Arabinose Transporters from Filamentous Fungi Reveals Promising Characteristics for Improved Pentose Utilization in Saccharomyces cerevisiae[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2015,81(12):4062-4070.
APA	Li, Jingen.,Xu, Jing.,Cai, Pengli.,Wang, Bang.,Ma, Yanhe.,...&Tian, Chaoguang.(2015).Functional Analysis of Two L-Arabinose Transporters from Filamentous Fungi Reveals Promising Characteristics for Improved Pentose Utilization in Saccharomyces cerevisiae.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,81(12),4062-4070.
MLA	Li, Jingen,et al."Functional Analysis of Two L-Arabinose Transporters from Filamentous Fungi Reveals Promising Characteristics for Improved Pentose Utilization in Saccharomyces cerevisiae".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 81.12(2015):4062-4070.