Insight into the transcriptional regulation of Msn2 required for conidiation, multi-stress responses and virulence of two entomopathogenic fungi

CORC > 天津工业生物技术研究所 > 微生物功能基因组学与生物技术实验室田朝光 > 期刊论文

	Insight into the transcriptional regulation of Msn2 required for conidiation, multi-stress responses and virulence of two entomopathogenic fungi
	Liu, Qian 1,2; Ying, Sheng-Hua 1; Li, Jin-Gen 2; Tian, Chao-Guang 2; Feng, Ming-Guang 1
刊名	FUNGAL GENETICS AND BIOLOGY
	2013-05-01
卷号	54 页码:42-51
关键词	Entomopathogenic fungi Msn2 transcription factor Regulation of gene expression Stress responses Virulence regulation Transcriptomes
英文摘要	Msn2/4 transcription factors in some fungi have null effects on virulence and cellular stress responses. Here we found that the transcriptional regulation of Msn2 orthologs is vital for the conidiation, virulence and multi-stress responses of Beauveria bassiana (Bb) and Metarhizium robertsii (Mr), which lack Msn4 orthologs. Compared to wild-type and complemented strains of each fungus with all similar phenotypes, Delta Bbmsn2 and Delta Mrmsn2 showed remarkable defects in conidial yield (similar to 40% decrease) and virulence (similar to 25% decrease). Both delta mutants lost 20-65% of their tolerances to hyperosmolarity, oxidation, carbendazim, cell wall perturbing and high temperature at 34 degrees C during colony growth. Their conidia were also significantly (18-41%) less tolerant to oxidation, hyperosmolarity, wet-heat stress at 45 degrees C and UV-B irradiation. Accompanied with the defective phenotypes, several conidiation- and virulence-associated genes were greatly repressed in Delta Bbmsn2 and Delta Mrmsn2. Moreover, differentially expressed genes in the transcriptomes of Delta Bbmsn2 versus wild type were similar to 3% more under oxidative stress, but similar to 12% fewer under heat shock, than those in the Delta Mrmsn2 counterparts. Many stress-responsive effector genes and cellular signaling factors were remarkably downregulated. Taken together, the two entomopathogens could have evolved somewhat distinct stress-responsive mechanisms finely tuned by Msn2, highlighting the biological significance of Msn2 orthologs for filamentous fungi. (C) 2013 Elsevier Inc. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Genetics & Heredity ; Mycology
研究领域[WOS]	Genetics & Heredity ; Mycology
关键词[WOS]	PATHOGEN CANDIDA-ALBICANS ; SACCHAROMYCES-CEREVISIAE ; BEAUVERIA-BASSIANA ; METARHIZIUM-ANISOPLIAE ; ASPERGILLUS-NIDULANS ; NUCLEAR-LOCALIZATION ; PROTEIN-KINASE ; ASEXUAL DEVELOPMENT ; GLUCOSE STARVATION ; YEAST
收录类别	SCI
语种	英语
WOS记录号	WOS:000319374100005
内容类型	期刊论文
源URL	[http://124.16.173.210/handle/834782/1256]
专题	天津工业生物技术研究所_微生物功能基因组学与生物技术实验室田朝光_期刊论文
作者单位	1.Zhejiang Univ, Coll Life Sci, Inst Microbiol, Hangzhou 310058, Zhejiang, Peoples R China 2.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China
推荐引用方式 GB/T 7714	Liu, Qian,Ying, Sheng-Hua,Li, Jin-Gen,et al. Insight into the transcriptional regulation of Msn2 required for conidiation, multi-stress responses and virulence of two entomopathogenic fungi[J]. FUNGAL GENETICS AND BIOLOGY,2013,54:42-51.
APA	Liu, Qian,Ying, Sheng-Hua,Li, Jin-Gen,Tian, Chao-Guang,&Feng, Ming-Guang.(2013).Insight into the transcriptional regulation of Msn2 required for conidiation, multi-stress responses and virulence of two entomopathogenic fungi.FUNGAL GENETICS AND BIOLOGY,54,42-51.
MLA	Liu, Qian,et al."Insight into the transcriptional regulation of Msn2 required for conidiation, multi-stress responses and virulence of two entomopathogenic fungi".FUNGAL GENETICS AND BIOLOGY 54(2013):42-51.