Production of lycopene by metabolically-engineered Escherichia coli

	Production of lycopene by metabolically-engineered Escherichia coli
	Sun, Tao 1,2,3; Miao, Liangtian 1,2,3; Li, Qingyan 2,3; Dai, Guanping 1,2,3; Lu, Fuping 1; Liu, Tao 2,3; Zhang, Xueli 2,3; Ma, Yanhe 2
刊名	BIOTECHNOLOGY LETTERS
	2014-07-01
卷号	36 期号:7 页码:1515-1522
关键词	beta-Carotene Escherichia coli Isoprenoid biosynthesis Lycopene NADPH Ribosome-binding sites
英文摘要	Escherichia coli strain CAR001 that produces beta-carotene was genetically engineered to produce lycopene by deleting genes encoding zeaxanthin glucosyltransferase (crtX) and lycopene beta-cyclase (crtY) from the crtEXYIB operon. The resulting strain, LYC001, produced 10.5 mg lycopene/l (6.5 mg/g dry cell weight, DCW). Modulating expression of genes encoding alpha-ketoglutarate dehydrogenase, succinate dehydrogenase and transaldolase B within central metabolic modules increased NADPH and ATP supplies, leading to a 76 % increase of lycopene yield. Ribosome binding site libraries were further used to modulate expression of genes encoding 1-deoxy-d-xylulose-5-phosphate synthase (dxs) and isopentenyl diphosphate isomerase (idi) and the crt gene operon, which improved the lycopene yield by 32 %. The optimal strain LYC010 produced 3.52 g lycopene/l (50.6 mg/g DCW) in fed-batch fermentation.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology
研究领域[WOS]	Biotechnology & Applied Microbiology
关键词[WOS]	BETA-CAROTENE ; E. COLI ; GENES ; BIOSYNTHESIS ; MEVALONATE ; STRAINS ; PATHWAY ; DISEASE
收录类别	SCI
语种	英语
WOS记录号	WOS:000337139500017
内容类型	期刊论文
源URL	[http://124.16.173.210/handle/834782/1391]
专题	天津工业生物技术研究所_微生物代谢工程张学礼_期刊论文
作者单位	1.Tianjin Univ Sci & Technol, Coll Biotechnol, Tianjin, Peoples R China 2.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China 3.Chinese Acad Sci, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China
推荐引用方式 GB/T 7714	Sun, Tao,Miao, Liangtian,Li, Qingyan,et al. Production of lycopene by metabolically-engineered Escherichia coli[J]. BIOTECHNOLOGY LETTERS,2014,36(7):1515-1522.
APA	Sun, Tao.,Miao, Liangtian.,Li, Qingyan.,Dai, Guanping.,Lu, Fuping.,...&Ma, Yanhe.(2014).Production of lycopene by metabolically-engineered Escherichia coli.BIOTECHNOLOGY LETTERS,36(7),1515-1522.
MLA	Sun, Tao,et al."Production of lycopene by metabolically-engineered Escherichia coli".BIOTECHNOLOGY LETTERS 36.7(2014):1515-1522.