Activating C-4-dicarboxylate transporters DcuB and DcuC for improving succinate production

	Activating C-4-dicarboxylate transporters DcuB and DcuC for improving succinate production
	Chen, Jing 1,2,3; Zhu, Xinna 2,3; Tan, Zaigao 2,3; Xu, Hongtao 2,3; Tang, Jinlei 2,3; Xiao, Dongguang 1; Zhang, Xueli 2,3
刊名	APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
	2014-03-01
卷号	98 期号:5 页码:2197-2205
关键词	DcuB DcuC Succinate Transporter Escherichia coli
英文摘要	Although many efforts had been performed to engineer Escherichia coli for succinate production, succinate efflux system had not been investigated as an engineering target for improving succinate production. In this work, four Dcu transporters, which had been reported to be responsible for C-4-dicarboxylates transportation of E. coli, were investigated for their succinate efflux capabilities. These four dcu genes were deleted individually in a previously constructed succinate-producing strain to study their effects on succinate production. Deleting dcuA and dcuD genes had nearly no influence, while deleting dcuB and dcuC genes led to 15 and 11 % decrease of succinate titer, respectively. Deleting both dcuB and dcuC genes resulted in 90 % decrease of succinate titer, suggesting that DcuB and DcuC were the main transporters for succinate efflux and they functioned as independent and mutually redundant succinate efflux transporters. Furthermore, RBS library having strengths varied from 0.17 to 8.6 times of induced E. coli lacZ promoter was used to modulate dcuB and dcuC genes for improving succinate production. Modulating these two genes in combination led to 34 % increase of succinate titer. To the best of knowledge, this was the first report about improving succinate production through engineering succinate efflux system.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology
研究领域[WOS]	Biotechnology & Applied Microbiology
关键词[WOS]	ENGINEERED ESCHERICHIA-COLI ; METABOLIC EVOLUTION ; GLUCOSE ; FERMENTATION ; EXPRESSION ; PATHWAYS ; STRAINS ; MUTANT ; YIELD ; ACID
收录类别	SCI
语种	英语
WOS记录号	WOS:000332107700025
内容类型	期刊论文
源URL	[http://124.16.173.210/handle/834782/1376]
专题	天津工业生物技术研究所_微生物代谢工程张学礼_期刊论文
作者单位	1.Tianjin Univ Sci Technol, Coll Biotechnol, Tianjin, Peoples R China 2.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China 3.Chinese Acad Sci, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China
推荐引用方式 GB/T 7714	Chen, Jing,Zhu, Xinna,Tan, Zaigao,et al. Activating C-4-dicarboxylate transporters DcuB and DcuC for improving succinate production[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2014,98(5):2197-2205.
APA	Chen, Jing.,Zhu, Xinna.,Tan, Zaigao.,Xu, Hongtao.,Tang, Jinlei.,...&Zhang, Xueli.(2014).Activating C-4-dicarboxylate transporters DcuB and DcuC for improving succinate production.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,98(5),2197-2205.
MLA	Chen, Jing,et al."Activating C-4-dicarboxylate transporters DcuB and DcuC for improving succinate production".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 98.5(2014):2197-2205.