Isolation and characterization of a novel Rhodococcus strain with switchable carbonyl reductase and para-acetylphenol hydroxylase activities

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	Isolation and characterization of a novel Rhodococcus strain with switchable carbonyl reductase and para-acetylphenol hydroxylase activities
	Zhang, Rui 1,2; Ren, Jie 1; Wang, Yu 1; Wu, Qiaqing 1; Wang, Min 2; Zhu, Dunming 1
刊名	JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
	2013
卷号	40 期号:1 页码:11-20
关键词	Phenol hydroxylase Carbonyl reductase Rhodococcus Hydroxylation Bioreduction
英文摘要	In the search for an effective biocatalyst for the reduction of acetophenones with unprotected hydroxy group on the benzene ring, a microorganism, which reduced para-acetylphenol to S-(-)-1-(para-hydroxyphenyl)ethanol under anaerobic conditions, was isolated from soil samples and the 16S rDNA study showed that it was phylogenetically affiliated with species of the genus Rhodococcus and was most similar to Rhodococcus pyridinivorans. Unexpectedly, this strain also hydroxylated para-acetylphenol to give 4-acetylcatechol in presence of oxygen, possessing para-acetylphenol hydroxylase activity. While the reduction of para-acetylphenol had an optimal reaction pH at 7 and a broad optimal temperature range (35-45 A degrees C), the hydroxylation reached the maximum conversion at the pH range of 7-8 and 35 A degrees C. This study identified for the first time a Rhodococcus strain with para-acetylphenol hydroxylase activity, which also contains highly enantioselective carbonyl reductase activity with potential applications for the asymmetric reduction of these less-explored but important ketones such as alpha-aminoacetophenone, 3'-hydroxyacetophenone and 4'-hydroxyacetophenone. The para-acetylphenol hydroxylase and carbonyl reductase activity are switchable by the reaction conditions.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology
研究领域[WOS]	Biotechnology & Applied Microbiology
关键词[WOS]	PSEUDOMONAS-FLUORESCENS ACB ; 4-HYDROXYACETOPHENONE MONOOXYGENASE ; ASYMMETRIC REDUCTION ; 4-NITROPHENOL HYDROXYLASE ; ASPERGILLUS-FUMIGATUS ; GENE-CLUSTER ; PUTIDA JD1 ; (R)-SALMETEROL ; IDENTIFICATION ; 4-ETHYLPHENOL
收录类别	SCI
语种	英语
WOS记录号	WOS:000313019300002
内容类型	期刊论文
源URL	[http://124.16.173.210/handle/834782/1252]
专题	天津工业生物技术研究所_生物催化与绿色化工朱敦明_期刊论文
作者单位	1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Natl Engn Lab Ind Enzymes, Tianjin 300308, Peoples R China 2.Tianjin Univ Sci & Technol, Coll Biotechnol, Minist Educ, Key Lab Ind Fermentat Microbiol, Tianjin 300457, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Rui,Ren, Jie,Wang, Yu,et al. Isolation and characterization of a novel Rhodococcus strain with switchable carbonyl reductase and para-acetylphenol hydroxylase activities[J]. JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,2013,40(1):11-20.
APA	Zhang, Rui,Ren, Jie,Wang, Yu,Wu, Qiaqing,Wang, Min,&Zhu, Dunming.(2013).Isolation and characterization of a novel Rhodococcus strain with switchable carbonyl reductase and para-acetylphenol hydroxylase activities.JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,40(1),11-20.
MLA	Zhang, Rui,et al."Isolation and characterization of a novel Rhodococcus strain with switchable carbonyl reductase and para-acetylphenol hydroxylase activities".JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY 40.1(2013):11-20.