Fermentative production of enantiomerically pure S-1,2-propanediol from glucose by engineered E. coli strain

	Fermentative production of enantiomerically pure S-1,2-propanediol from glucose by engineered E. coli strain
	Zhu, Lingfeng 1,4; Guan, Xiangchen 1; Xie, Nengzhong 3; Wang, Limin 1; Yu, Bo 1; Ma, Yanhe 2
刊名	APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
	2016-02-01
卷号	100 期号:3 页码:1241-1251
关键词	S-1,2-propanediol L-lactate Glucose Metabolic engineering Fermentation
英文摘要	The pure stereoisomers of 1,2-propanediol (1,2-PDO) could be used as starting materials to synthesize high value-added specialty chemicals and chiral pharmaceutical products. As the stereoisomers of 1,2-PDO cannot be obtained by traditional chemical synthesis processes, biotechnological processes have gained increasing attention. However, to our knowledge, the production of S-1,2-PDO directly from glucose has not been previously reported. In this study, we demonstrate a novel artificial pathway to convert L-lactic acid to S1,2- PDO and its integration into the genome of Escherichia coli strain BW25113 Delta poxB with synchronous deletion of genes responsible for branch metabolic pathways from glucose. L-lactate production was increased by replacing the native D-lactate dehydrogenase with the L-lactate dehydrogenase from Bacillus coagulans. The methylglyoxal bypass pathway was blocked to avoid synthesis of a racemic mixture of D-and L-lactate and prevent the accumulation of methylglyoxal, a toxic intermediate. To further improve the yield of S-1,2PDO, a novel cofactor regeneration system was introduced by combining pyruvate decarboxylase and acetaldehyde-CoA dehydrogenase II to simultaneously regenerate NADH and the CoA donor of acetyl-CoA for the lactate conversion pathway. Finally, 13.7 mM S-1,2-PDO with >99 % enantiomeric purity was directly produced from glucose by disrupting the major carbon-competing pathways and strengthening the lactate transformation pathway. This study demonstrates the first attempt to synthesize S-1,2-PDO by direct fermentation of glucose.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology
研究领域[WOS]	Biotechnology & Applied Microbiology
关键词[WOS]	ESCHERICHIA-COLI ; LACTOBACILLUS-BUCHNERI ; LACTIC-ACID ; CLOSTRIDIUM-THERMOSACCHAROLYTICUM ; 1,2-PROPANEDIOL PRODUCTION ; EFFICIENT PRODUCTION ; ENHANCED PRODUCTION ; L-RHAMNOSE ; L-LACTATE ; DEHYDROGENASE
收录类别	SCI
语种	英语
WOS记录号	WOS:000369308900016
内容类型	期刊论文
源URL	[http://124.16.173.210/handle/834782/1544]
专题	天津工业生物技术研究所_酶工程实验室马延和 _期刊论文
作者单位	1.Chinese Acad Sci, Inst Microbiol, Key Lab Microbial Physiol & Metab Engn, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China 3.Guangxi Acad Sci, Natl Engn Res Ctr Nonfood Biorefinery, Nanning 530007, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhu, Lingfeng,Guan, Xiangchen,Xie, Nengzhong,et al. Fermentative production of enantiomerically pure S-1,2-propanediol from glucose by engineered E. coli strain[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2016,100(3):1241-1251.
APA	Zhu, Lingfeng,Guan, Xiangchen,Xie, Nengzhong,Wang, Limin,Yu, Bo,&Ma, Yanhe.(2016).Fermentative production of enantiomerically pure S-1,2-propanediol from glucose by engineered E. coli strain.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,100(3),1241-1251.
MLA	Zhu, Lingfeng,et al."Fermentative production of enantiomerically pure S-1,2-propanediol from glucose by engineered E. coli strain".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 100.3(2016):1241-1251.