Identification and characterization of a novel salt-tolerant esterase from a Tibetan glacier metagenomic library

	Identification and characterization of a novel salt-tolerant esterase from a Tibetan glacier metagenomic library
	De Santi, Concetta 1; Ambrosino, Luca 1; Tedesco, Pietro 1; de Pascale, Donatella 1; Zhai, Lei 2,3,4; Zhou, Cheng 2,3; Xue, Yanfen 2,3; Ma, Yanhe 2,3
刊名	BIOTECHNOLOGY PROGRESS
	2015-07-01
卷号	31 期号:4 页码:890-899
关键词	metagenomic esterase salt-tolerant cold-tolerant
英文摘要	A salt-tolerant esterase, designated H9Est, was identified from a metagenomic library of the Karuola glacier. H9Est gene comprised 1071 bp and encoded a polypeptide of 357 amino acids with a molecular mass of 40 kDa. Sequence analysis revealed that H9Est belonged to the family IV of bacterial lypolitic enzyme. H9Est was overexpressed in Escherichia coli and the purified enzyme showed hydrolytic activity towards p-nitrophenyl esters with carbon chain from 2 to 8. The optimal esterase activity was at 40 degrees C and pH 8.0 and the enzyme retained its activity towards some miscible organic solvents such as polyethylene glycol. A three-dimensional model of H9Est revealed that S200, D294, and H324 formed the H9Est catalytic triad. Circular Dichroism spectra and molecular dynamic simulation indicated that the esterase had a wide denaturation temperature range and flexible loops that would be beneficial for H9Est performance at low temperatures while retaining heat-resistant features. (c) 2015 American Institute of Chemical Engineers Biotechnol. Prog., 31:890-899, 2015
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology ; Food Science & Technology
研究领域[WOS]	Biotechnology & Applied Microbiology ; Food Science & Technology
关键词[WOS]	SOUTH CHINA SEA ; LIPOLYTIC GENES ; UNCULTURED BACTERIA ; SEDIMENT METAGENOME ; CARBOXYL ESTERASES ; MARINE SEDIMENT ; ENZYMES ; LIPASE ; CLONING ; SOIL
收录类别	SCI
语种	英语
WOS记录号	WOS:000359602900005
内容类型	期刊论文
源URL	[http://124.16.173.210/handle/834782/1511]
专题	天津工业生物技术研究所_酶工程实验室马延和 _期刊论文
作者单位	1.CNR, Inst Prot Biochem, I-80131 Naples, Italy 2.Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100101, Peoples R China 3.Chinese Acad Sci, Inst Microbiol, Natl Engn Lab Ind Enzymes, Beijing 100101, Peoples R China 4.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
推荐引用方式 GB/T 7714	De Santi, Concetta,Ambrosino, Luca,Tedesco, Pietro,et al. Identification and characterization of a novel salt-tolerant esterase from a Tibetan glacier metagenomic library[J]. BIOTECHNOLOGY PROGRESS,2015,31(4):890-899.
APA	De Santi, Concetta.,Ambrosino, Luca.,Tedesco, Pietro.,de Pascale, Donatella.,Zhai, Lei.,...&Ma, Yanhe.(2015).Identification and characterization of a novel salt-tolerant esterase from a Tibetan glacier metagenomic library.BIOTECHNOLOGY PROGRESS,31(4),890-899.
MLA	De Santi, Concetta,et al."Identification and characterization of a novel salt-tolerant esterase from a Tibetan glacier metagenomic library".BIOTECHNOLOGY PROGRESS 31.4(2015):890-899.