Genetic diversity and expression profiles of cysteine phytases in the sheep rumen during a feeding cycle

	Genetic diversity and expression profiles of cysteine phytases in the sheep rumen during a feeding cycle
	Li, Z.1,2; Huang, H.1; Zhao, H.1; Meng, K.1; Zhao, J.3; Shi, P.1; Yang, P.1; Luo, H.1; Wang, Y.1; Yao, B.1
刊名	LETTERS IN APPLIED MICROBIOLOGY
	2014-12-01
卷号	59 期号:6 页码:615-620
关键词	clone library cysteine phytase rumen Small Tail Han sheep transcriptional profile
英文摘要	Cysteine phytase is the main phytate-degrading enzyme of ruminant animals. To explore the genetic diversity and dynamic expression profile of cysteine phytase in sheep rumen during a feeding cycle, four transcript (0, 4, 9 and 16h after feeding) and one DNA (9h after feeding) clone libraries were constructed, respectively. A total of 46 distinct gene fragments were identified, and most of these sequences had low identities (<60%) with known phytases. Great divergence was found in the constitution and abundance of genes at the genome and transcriptional levels, and the transcript data are more reliable to reflect the information of functional genes. Phylogenetic analysis indicated that the genes from uncultured bacteria instead of Firmicutes played the major phytate-degrading role. Further comparative analysis revealed the dynamic constitution of cysteine phytase genes in rumen at different time points.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology ; Microbiology
研究领域[WOS]	Biotechnology & Applied Microbiology ; Microbiology
关键词[WOS]	PROGRAM ; PROTEIN
收录类别	SCI
语种	英语
WOS记录号	WOS:000345218100008
内容类型	期刊论文
源URL	[http://124.16.173.210/handle/834782/1388]
专题	天津工业生物技术研究所_酶工程实验室马延和 _期刊论文
作者单位	1.Chinese Acad Agr Sci, Feed Res Inst, Minist Agr, Key Lab Feed Biotechnol, Beijing 100081, Peoples R China 2.Tianjin Univ Sci & Technol, Key Lab Ind Fermentat Microbiol, Tianjin, Peoples R China 3.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Natl Engn Lab Ind Enzymes, Tianjin, Peoples R China
推荐引用方式 GB/T 7714	Li, Z.,Huang, H.,Zhao, H.,et al. Genetic diversity and expression profiles of cysteine phytases in the sheep rumen during a feeding cycle[J]. LETTERS IN APPLIED MICROBIOLOGY,2014,59(6):615-620.
APA	Li, Z..,Huang, H..,Zhao, H..,Meng, K..,Zhao, J..,...&Yao, B..(2014).Genetic diversity and expression profiles of cysteine phytases in the sheep rumen during a feeding cycle.LETTERS IN APPLIED MICROBIOLOGY,59(6),615-620.
MLA	Li, Z.,et al."Genetic diversity and expression profiles of cysteine phytases in the sheep rumen during a feeding cycle".LETTERS IN APPLIED MICROBIOLOGY 59.6(2014):615-620.