Crystal structure and substrate-binding mode of the mycoestrogen-detoxifying lactonase ZHD from Clonostachys rosea

CORC > 天津工业生物技术研究所 > 结构生物学与蛋白酶学实验室郭瑞庭 > 期刊论文

	Crystal structure and substrate-binding mode of the mycoestrogen-detoxifying lactonase ZHD from Clonostachys rosea
	Peng, Wei 1,2; Ko, Tzu-Ping 3; Yang, Yunyun 1,2; Zheng, Yingying 2; Chen, Chun-Chi 2; Zhu, Zhen 2; Huang, Chun-Hsiang 2; Zeng, Yi-Fang 3; Huang, Jian-Wen 7,8; Wang, Andrew H. -J.3
刊名	RSC ADVANCES
	2014
卷号	4 期号:107 页码:62321-62325
英文摘要	The mycotoxin zearalenone has been contaminating maize and other grains. It can be hydrolyzed and inactivated by the lactonase ZHD, which belongs to the alpha/beta-hydrolase family. Besides the catalytic core domain, the enzyme comprises an alpha-helical cap domain. Zearalenone differs from other quorum-sensing lactones in its chemical structure. As revealed by the complex structure, the substrate binds into a deep pocket between the core and cap domains, adjacent to the catalytic triad Ser102-His242-Glu126. The enzyme-substrate interactions include three direct hydrogen bonds and several nonpolar contacts. In particular, the Trp183 side chain is engaged in both hydrogen bonding and T-stacking interactions with the benzoate ring. The central role of Trp183 in substrate binding was verified by the mutants W183A, W183H and W183F. Severalmutants were also produced to investigate the roles of nearby amino-acid residues. Interestingly, mutants that destabilize the dimer had adverse functional effects on ZHD.
WOS标题词	Science & Technology ; Physical Sciences
类目[WOS]	Chemistry, Multidisciplinary
研究领域[WOS]	Chemistry
关键词[WOS]	OXIDIZED POLYVINYL-ALCOHOL ; ZEARALENONE ; MYCOTOXINS ; DETOXIFICATION ; HYDROLASE ; INSIGHTS ; GENE
收录类别	SCI
语种	英语
WOS记录号	WOS:000345660300037
内容类型	期刊论文
源URL	[http://124.16.173.210/handle/834782/1343]
专题	天津工业生物技术研究所_结构生物学与蛋白酶学实验室郭瑞庭_期刊论文
作者单位	1.Tianjin Univ Sci & Technol, Coll Biotechnol, Tianjin 300457, Peoples R China 2.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China 3.Acad Sinica, Inst Biol Chem, Taipei 115, Taiwan 4.Acad Sinica, Agr Biotechnol Res Ctr, Taipei 115, Taiwan 5.Natl Taiwan Univ, Inst Biotechnol, Taipei 106, Taiwan 6.Natl Taiwan Univ, Dept Anim Sci & Technol, Taipei 106, Taiwan 7.Genozyme Biotechnol Inc, Taipei 106, Taiwan 8.AsiaPac Biotechnol Co Ltd, Dongguan 523808, Peoples R China
推荐引用方式 GB/T 7714	Peng, Wei,Ko, Tzu-Ping,Yang, Yunyun,et al. Crystal structure and substrate-binding mode of the mycoestrogen-detoxifying lactonase ZHD from Clonostachys rosea[J]. RSC ADVANCES,2014,4(107):62321-62325.
APA	Peng, Wei.,Ko, Tzu-Ping.,Yang, Yunyun.,Zheng, Yingying.,Chen, Chun-Chi.,...&Guo, Rey-Ting.(2014).Crystal structure and substrate-binding mode of the mycoestrogen-detoxifying lactonase ZHD from Clonostachys rosea.RSC ADVANCES,4(107),62321-62325.
MLA	Peng, Wei,et al."Crystal structure and substrate-binding mode of the mycoestrogen-detoxifying lactonase ZHD from Clonostachys rosea".RSC ADVANCES 4.107(2014):62321-62325.