Preliminary X-ray diffraction analysis of thermostable beta-1,4-mannanase from Aspergillus niger BK01

CORC > 天津工业生物技术研究所 > 结构生物学与蛋白酶学实验室郭瑞庭 > 期刊论文

	Preliminary X-ray diffraction analysis of thermostable beta-1,4-mannanase from Aspergillus niger BK01
	Luo, Wenhua 1; Huang, Jian-Wen 2; Huang, Chun-Hsiang 3; Huang, Ting-Yung 4,5; Chan, Hsiu-Chien 3; Liu, Je-Ruei 2; Guo, Rey-Ting 3; Chen, Chun-Chi 3
刊名	ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS
	2013-10-01
卷号	69 页码:1100-1102
英文摘要	beta-1,4-Mannanase (beta-mannanase) is a key enzyme in decomposing mannans, which are abundant components of hemicelluloses in the plant cell wall. Therefore, mannan hydrolysis is highly valuable in a wide array of industrial applications. beta-Mannanase isolated from Aspergillus niger BK01 (ManBK) was classified into glycoside hydrolase family GH5. ManBK holds great potential in biotechnological applications owing to its high thermostability. Here, ManBK was expressed and purified in Pichia pastoris and the recombinant protein was crystallized. Crystals belonging to the orthorhombic space group C222(1), with unit-cell parameters a = 93.58, b = 97.05, c = 147.84 angstrom, were obtained by the sitting-drop vapour-diffusion method and diffracted to 1.57 angstrom resolution. Structure determination using molecular-replacement methods is in progress.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]	Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biophysics ; Crystallography
研究领域[WOS]	Biochemistry & Molecular Biology ; Biophysics ; Crystallography
收录类别	SCI
语种	英语
WOS记录号	WOS:000325402900007
内容类型	期刊论文
源URL	[http://124.16.173.210/handle/834782/1281]
专题	天津工业生物技术研究所_结构生物学与蛋白酶学实验室郭瑞庭_期刊论文
作者单位	1.South China Agr Univ, Coll Food Sci, Guangzhou 510642, Guangdong, Peoples R China 2.Natl Taiwan Univ, Inst Biotechnol, Taipei 106, Taiwan 3.Chinese Acad Sci, Ind Enzymes Natl Engn Lab, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China 4.Genozyme Biotechnol Inc, Taipei 106, Taiwan 5.AsiaPac Biotechnol Co Ltd, Dongguan 523808, Peoples R China
推荐引用方式 GB/T 7714	Luo, Wenhua,Huang, Jian-Wen,Huang, Chun-Hsiang,et al. Preliminary X-ray diffraction analysis of thermostable beta-1,4-mannanase from Aspergillus niger BK01[J]. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS,2013,69:1100-1102.
APA	Luo, Wenhua.,Huang, Jian-Wen.,Huang, Chun-Hsiang.,Huang, Ting-Yung.,Chan, Hsiu-Chien.,...&Chen, Chun-Chi.(2013).Preliminary X-ray diffraction analysis of thermostable beta-1,4-mannanase from Aspergillus niger BK01.ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS,69,1100-1102.
MLA	Luo, Wenhua,et al."Preliminary X-ray diffraction analysis of thermostable beta-1,4-mannanase from Aspergillus niger BK01".ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS 69(2013):1100-1102.