Preliminary X-ray diffraction analysis of thermostable beta-1,4-mannanase from Aspergillus niger BK01 | |
Luo, Wenhua1; Huang, Jian-Wen2; Huang, Chun-Hsiang3; Huang, Ting-Yung4,5; Chan, Hsiu-Chien3; Liu, Je-Ruei2; Guo, Rey-Ting3; Chen, Chun-Chi3 | |
刊名 | ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS
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2013-10-01 | |
卷号 | 69页码:1100-1102 |
英文摘要 | beta-1,4-Mannanase (beta-mannanase) is a key enzyme in decomposing mannans, which are abundant components of hemicelluloses in the plant cell wall. Therefore, mannan hydrolysis is highly valuable in a wide array of industrial applications. beta-Mannanase isolated from Aspergillus niger BK01 (ManBK) was classified into glycoside hydrolase family GH5. ManBK holds great potential in biotechnological applications owing to its high thermostability. Here, ManBK was expressed and purified in Pichia pastoris and the recombinant protein was crystallized. Crystals belonging to the orthorhombic space group C222(1), with unit-cell parameters a = 93.58, b = 97.05, c = 147.84 angstrom, were obtained by the sitting-drop vapour-diffusion method and diffracted to 1.57 angstrom resolution. Structure determination using molecular-replacement methods is in progress. |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
类目[WOS] | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biophysics ; Crystallography |
研究领域[WOS] | Biochemistry & Molecular Biology ; Biophysics ; Crystallography |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000325402900007 |
内容类型 | 期刊论文 |
源URL | [http://124.16.173.210/handle/834782/1281] ![]() |
专题 | 天津工业生物技术研究所_结构生物学与蛋白酶学实验室 郭瑞庭_期刊论文 |
作者单位 | 1.South China Agr Univ, Coll Food Sci, Guangzhou 510642, Guangdong, Peoples R China 2.Natl Taiwan Univ, Inst Biotechnol, Taipei 106, Taiwan 3.Chinese Acad Sci, Ind Enzymes Natl Engn Lab, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China 4.Genozyme Biotechnol Inc, Taipei 106, Taiwan 5.AsiaPac Biotechnol Co Ltd, Dongguan 523808, Peoples R China |
推荐引用方式 GB/T 7714 | Luo, Wenhua,Huang, Jian-Wen,Huang, Chun-Hsiang,et al. Preliminary X-ray diffraction analysis of thermostable beta-1,4-mannanase from Aspergillus niger BK01[J]. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS,2013,69:1100-1102. |
APA | Luo, Wenhua.,Huang, Jian-Wen.,Huang, Chun-Hsiang.,Huang, Ting-Yung.,Chan, Hsiu-Chien.,...&Chen, Chun-Chi.(2013).Preliminary X-ray diffraction analysis of thermostable beta-1,4-mannanase from Aspergillus niger BK01.ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS,69,1100-1102. |
MLA | Luo, Wenhua,et al."Preliminary X-ray diffraction analysis of thermostable beta-1,4-mannanase from Aspergillus niger BK01".ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS 69(2013):1100-1102. |
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