Mitochondria thioredoxin's backup role in oxidative stress resistance in Trichoderma reesei

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	Mitochondria thioredoxin's backup role in oxidative stress resistance in Trichoderma reesei
	Wang, Guokun 1,2; Wang, Haijun 1; Xiong, Xiaochao 3; Chen, Shulin 1; Zhang, Dongyuan 1
刊名	MICROBIOLOGICAL RESEARCH
	2015
卷号	171 页码:32-38
关键词	Glutathione Mitochondria Reactive oxygen species Thioredoxin Trichoderma reesei
英文摘要	Microorganisms often suffer from oxidative stress created from nutrient starvation and environmental changes. Thioredoxin (Trx) and glutathione (GSH) pathways are believed critical in related protective functions. The roles of Trx in improving abiotic stress resistance in Trichoderma reesei are still unclear. In this study, we identified a Trx-encoding gene, Trtrx1. The protein expressed located specifically in the mitochondria as verified by the fluorescence signals of TrTRX1-EGFP. TrTRX1 can catalyze the reduction of insulin disulfides by dithiothreitol (DTT). Loss of Trtrx1 however, did not lead to either significant morphology abnormality under normal and oxidative stress condition, or detectable difference in reactive oxygen species (ROS) resistance. The unchanged GSH amount in Trtrx1 deletion strain under normal condition and slight increase under oxidative stress condition, as well as the interplay between Trx and GSH systems suggested that GSH system was dominant and sufficient to maintain the mitochondrial redox state in T. reesei, where TrTRX1 played a role as the backup oxidative stress resistance. (C) 2015 Elsevier GmbH. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Microbiology
研究领域[WOS]	Microbiology
关键词[WOS]	YEAST SACCHAROMYCES-CEREVISIAE ; HYDROGEN-PEROXIDE ; TRANSFORMATION SYSTEM ; FILAMENTOUS FUNGI ; GLUTATHIONE ; PROTECTION ; STARVATION ; REDUCTION ; EFFICIENT ; RESPONSES
收录类别	SCI
语种	英语
WOS记录号	WOS:000350083900005
内容类型	期刊论文
源URL	[http://124.16.173.210/handle/834782/1432]
专题	天津工业生物技术研究所_工业生物系统工程实验室陈树林_期刊论文
作者单位	1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin Key Lab Ind Biosyst & Bioproc Engn, Tianjin 300308, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Washington State Univ, Dept Biol Syst Engn, Pullman, WA 99164 USA
推荐引用方式 GB/T 7714	Wang, Guokun,Wang, Haijun,Xiong, Xiaochao,et al. Mitochondria thioredoxin's backup role in oxidative stress resistance in Trichoderma reesei[J]. MICROBIOLOGICAL RESEARCH,2015,171:32-38.
APA	Wang, Guokun,Wang, Haijun,Xiong, Xiaochao,Chen, Shulin,&Zhang, Dongyuan.(2015).Mitochondria thioredoxin's backup role in oxidative stress resistance in Trichoderma reesei.MICROBIOLOGICAL RESEARCH,171,32-38.
MLA	Wang, Guokun,et al."Mitochondria thioredoxin's backup role in oxidative stress resistance in Trichoderma reesei".MICROBIOLOGICAL RESEARCH 171(2015):32-38.