Biochemical characterization of a new beta-glucosidase (Cel3E) from Penicillium piceum and its application in boosting lignocelluloses bioconversion and forming disaccharide inducers: New insights into the role of beta-glucosidase

CORC > 天津工业生物技术研究所 > 工业生物系统工程实验室陈树林 > 期刊论文

	Biochemical characterization of a new beta-glucosidase (Cel3E) from Penicillium piceum and its application in boosting lignocelluloses bioconversion and forming disaccharide inducers: New insights into the role of beta-glucosidase
	Gao, Le 1; Gao, Feng 1; Jiang, Xukai 2; Zhang, Can 1; Zhang, Dongyuan 1; Wang, Lushan 2; Wu, Gaihong 1; Chen, Shulin 1
刊名	PROCESS BIOCHEMISTRY
	2014-05-01
卷号	49 期号:5 页码:768-774
关键词	beta-1,4-glucosidase PpCel3E Inducer Synergism Transglycosylation
英文摘要	Fungal genome sequencing has revealed the presence of multiple putative beta-glucosidases; however, information regarding these new beta-glucosidases is limited. A new beta-glucosidase from Penicillium piceum, designated as PpCel3E, was first isolated and characterized. Using p-nitrophenyl-beta-D-glucoside as substrate, PpCel3E showed the lowest Km among the P-glucosidases among all fungi studied. Moreover, PpCel3E exhibited a high transglycosylation activity of 1100mg gentiobiose/mg and 142 mg sophorose/mg using glucose as the donor. PpCel3E is a novel bifunctional glycoside hydrolase with both P-glucosidase and P-xylosidase activity. Our results show that PpCel3E plays an important role in forming soluble cellulose inducer compounds, as well as in amplifying weak cellulase inducer signal and hemicellulase synthesis via its high transglycosylation activity. Supplementing PpCel3E at low concentrations (40 mu g/g substrate) increased the saccharification efficiency of different cellulases by 20% to 27% by removing multiple inhibitors. 2014 Elsevier Ltd. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Technology
类目[WOS]	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering, Chemical
研究领域[WOS]	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering
关键词[WOS]	TRICHODERMA-REESEI ; TALAROMYCES-EMERSONII ; FILAMENTOUS FUNGUS ; MOLECULAR-CLONING ; DEGRADING ENZYMES ; HYPOCREA-JECORINA ; CELLULASE ; CELLOBIOHYDROLASE ; GENE ; PURIFICATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000336111700007
内容类型	期刊论文
源URL	[http://124.16.173.210/handle/834782/1416]
专题	天津工业生物技术研究所_工业生物系统工程实验室陈树林_期刊论文
作者单位	1.Chinese Acad Sci, Inst Ind Biotechnol, Tianjin Key Lab Ind Biol Syst & Bioproc Eng, Tianjin, Peoples R China 2.Shandong Univ, State Key Lab Microbial Technol, Jinan 250100, Peoples R China
推荐引用方式 GB/T 7714	Gao, Le,Gao, Feng,Jiang, Xukai,et al. Biochemical characterization of a new beta-glucosidase (Cel3E) from Penicillium piceum and its application in boosting lignocelluloses bioconversion and forming disaccharide inducers: New insights into the role of beta-glucosidase[J]. PROCESS BIOCHEMISTRY,2014,49(5):768-774.
APA	Gao, Le.,Gao, Feng.,Jiang, Xukai.,Zhang, Can.,Zhang, Dongyuan.,...&Chen, Shulin.(2014).Biochemical characterization of a new beta-glucosidase (Cel3E) from Penicillium piceum and its application in boosting lignocelluloses bioconversion and forming disaccharide inducers: New insights into the role of beta-glucosidase.PROCESS BIOCHEMISTRY,49(5),768-774.
MLA	Gao, Le,et al."Biochemical characterization of a new beta-glucosidase (Cel3E) from Penicillium piceum and its application in boosting lignocelluloses bioconversion and forming disaccharide inducers: New insights into the role of beta-glucosidase".PROCESS BIOCHEMISTRY 49.5(2014):768-774.