High Production of beta-Glucosidase by Aspergillus niger on Corncob | |
Wang, Chunli1,2; Wu, Gaihong2; Chen, Chang1; Chen, Shulin3 | |
刊名 | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
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2012-09-01 | |
卷号 | 168期号:1页码:58-67 |
关键词 | Corncob beta-Glucosidase Aspergillus niger Xylan The water-soluble components Water-insoluble components |
英文摘要 | Using low-cost raw material is an effective approach for reducing the cost of cellulolytic enzymes. The farmland waste corncob was found in this study to be the best carbon source for the production of beta-glucosidase by Aspergillus niger. The maximum yield of beta-glucosidase activity was 48.7 IU ml(-1) by using 50 g l(-1) of corncob powder as the substrate. It was found that the water-soluble components of the corncob could increase beta-glucosidase production significantly only when mixed with Avicel or wheat bran. The soluble components could not enhance the biomass and beta-glucosidase production when used alone. On the other hand, the water-insoluble components of the corncob still produced high level of beta-glucosidase (30 IU ml(-1)) although lower than that of using whole corncob. The results suggested that the water-insoluble components of corncob were beneficial for beta-glucosidase production. It was further demonstrated that the xylan in the water-insoluble parts of corncob was the important factor in producing beta-glucosidase by A. niger. |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
类目[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
研究领域[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
关键词[WOS] | TRICHODERMA-REESEI ; CARBON SOURCE ; WHEAT-FLOUR ; INDUCTION ; PURIFICATION ; CULTURE ; STRAINS ; XYLAN ; BRAN |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000309223700007 |
内容类型 | 期刊论文 |
源URL | [http://124.16.173.210/handle/834782/1319] ![]() |
专题 | 天津工业生物技术研究所_工业生物系统工程实验室 陈树林_期刊论文 |
作者单位 | 1.Beijing Univ Chem Technol, Coll Life Sci & Technol, Beijing 100029, Peoples R China 2.Chinese Acad Sci, Inst Microbiol, Beijing 100101, Peoples R China 3.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China |
推荐引用方式 GB/T 7714 | Wang, Chunli,Wu, Gaihong,Chen, Chang,et al. High Production of beta-Glucosidase by Aspergillus niger on Corncob[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2012,168(1):58-67. |
APA | Wang, Chunli,Wu, Gaihong,Chen, Chang,&Chen, Shulin.(2012).High Production of beta-Glucosidase by Aspergillus niger on Corncob.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,168(1),58-67. |
MLA | Wang, Chunli,et al."High Production of beta-Glucosidase by Aspergillus niger on Corncob".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 168.1(2012):58-67. |
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