Co-expression of D-glucose isomerase and D-psicose 3-epimerase: Development of an efficient one-step production of D-psicose | |
Men, Yan; Zhu, Yueming; Zeng, Yan; Izumori, Ken; Sun, Yuanxia; Ma, Yanhe | |
刊名 | ENZYME AND MICROBIAL TECHNOLOGY
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2014-10-01 | |
卷号 | 64-65页码:1-5 |
关键词 | Co-expression D-Glucose isomerase D-Psicose 3-epimerase D-Psicose High-fructose corn syru p(HFCS) |
英文摘要 | D-Psicose has been attracting attention in recent years because of its alimentary activities and is used as an ingredient in a range of foods and dietary supplements. To develop a one-step enzymatic process of D-psicose production, thermoactive D-glucose isomerase and the D-psicose 3-epimerase obtained from Bacillus sp. and Ruminococcus sp., respectively, were successfully co-expressed in Escherichia coli BL21 strain. The substrate of one-step enzymatic process was D-glucose. The co-expression system exhibited maximum activity at 65 degrees C and pH 7.0. Mg2+ could enhance the output of D-psicose by 232 fold to 1.6 g/L from 10 g/L of D-glucose. When using high-fructose corn syrup (HFCS) as substrate, 135 g/L D-psicose was produced under optimum conditions. The mass ratio of D-glucose, D-fructose, and D-psicose was almost 3.0:2.7:1.0, when the reaction reached equilibrium after an 8 h incubation time. This co-expression system approaching to produce D-psicose has potential application in food and beverage products, especially softdrinks. (C) 2014 Elsevier Inc. All rights reserved. |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
类目[WOS] | Biotechnology & Applied Microbiology |
研究领域[WOS] | Biotechnology & Applied Microbiology |
关键词[WOS] | D-TAGATOSE 3-EPIMERASE ; MAILLARD REACTION ; ESCHERICHIA-COLI ; D-FRUCTOSE ; RATS ; OVEREXPRESSION ; EXPRESSION ; COMPLEXES ; INGESTION ; MASS |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000342257000001 |
内容类型 | 期刊论文 |
源URL | [http://124.16.173.210/handle/834782/1405] ![]() |
专题 | 天津工业生物技术研究所_功能糖与天然活性物质研究组 孙媛霞 _期刊论文 |
作者单位 | Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Natl Engn Lab Ind Enzymes, Tianjin 300308, Peoples R China |
推荐引用方式 GB/T 7714 | Men, Yan,Zhu, Yueming,Zeng, Yan,et al. Co-expression of D-glucose isomerase and D-psicose 3-epimerase: Development of an efficient one-step production of D-psicose[J]. ENZYME AND MICROBIAL TECHNOLOGY,2014,64-65:1-5. |
APA | Men, Yan,Zhu, Yueming,Zeng, Yan,Izumori, Ken,Sun, Yuanxia,&Ma, Yanhe.(2014).Co-expression of D-glucose isomerase and D-psicose 3-epimerase: Development of an efficient one-step production of D-psicose.ENZYME AND MICROBIAL TECHNOLOGY,64-65,1-5. |
MLA | Men, Yan,et al."Co-expression of D-glucose isomerase and D-psicose 3-epimerase: Development of an efficient one-step production of D-psicose".ENZYME AND MICROBIAL TECHNOLOGY 64-65(2014):1-5. |
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