Co-expression of D-glucose isomerase and D-psicose 3-epimerase: Development of an efficient one-step production of D-psicose

CORC > 天津工业生物技术研究所 > 功能糖与天然活性物质研究组孙媛霞 > 期刊论文

	Co-expression of D-glucose isomerase and D-psicose 3-epimerase: Development of an efficient one-step production of D-psicose
	Men, Yan; Zhu, Yueming; Zeng, Yan; Izumori, Ken; Sun, Yuanxia; Ma, Yanhe
刊名	ENZYME AND MICROBIAL TECHNOLOGY
	2014-10-01
卷号	64-65 页码:1-5
关键词	Co-expression D-Glucose isomerase D-Psicose 3-epimerase D-Psicose High-fructose corn syru p(HFCS)
英文摘要	D-Psicose has been attracting attention in recent years because of its alimentary activities and is used as an ingredient in a range of foods and dietary supplements. To develop a one-step enzymatic process of D-psicose production, thermoactive D-glucose isomerase and the D-psicose 3-epimerase obtained from Bacillus sp. and Ruminococcus sp., respectively, were successfully co-expressed in Escherichia coli BL21 strain. The substrate of one-step enzymatic process was D-glucose. The co-expression system exhibited maximum activity at 65 degrees C and pH 7.0. Mg2+ could enhance the output of D-psicose by 232 fold to 1.6 g/L from 10 g/L of D-glucose. When using high-fructose corn syrup (HFCS) as substrate, 135 g/L D-psicose was produced under optimum conditions. The mass ratio of D-glucose, D-fructose, and D-psicose was almost 3.0:2.7:1.0, when the reaction reached equilibrium after an 8 h incubation time. This co-expression system approaching to produce D-psicose has potential application in food and beverage products, especially softdrinks. (C) 2014 Elsevier Inc. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology
研究领域[WOS]	Biotechnology & Applied Microbiology
关键词[WOS]	D-TAGATOSE 3-EPIMERASE ; MAILLARD REACTION ; ESCHERICHIA-COLI ; D-FRUCTOSE ; RATS ; OVEREXPRESSION ; EXPRESSION ; COMPLEXES ; INGESTION ; MASS
收录类别	SCI
语种	英语
WOS记录号	WOS:000342257000001
内容类型	期刊论文
源URL	[http://124.16.173.210/handle/834782/1405]
专题	天津工业生物技术研究所_功能糖与天然活性物质研究组孙媛霞 _期刊论文
作者单位	Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Natl Engn Lab Ind Enzymes, Tianjin 300308, Peoples R China
推荐引用方式 GB/T 7714	Men, Yan,Zhu, Yueming,Zeng, Yan,et al. Co-expression of D-glucose isomerase and D-psicose 3-epimerase: Development of an efficient one-step production of D-psicose[J]. ENZYME AND MICROBIAL TECHNOLOGY,2014,64-65:1-5.
APA	Men, Yan,Zhu, Yueming,Zeng, Yan,Izumori, Ken,Sun, Yuanxia,&Ma, Yanhe.(2014).Co-expression of D-glucose isomerase and D-psicose 3-epimerase: Development of an efficient one-step production of D-psicose.ENZYME AND MICROBIAL TECHNOLOGY,64-65,1-5.
MLA	Men, Yan,et al."Co-expression of D-glucose isomerase and D-psicose 3-epimerase: Development of an efficient one-step production of D-psicose".ENZYME AND MICROBIAL TECHNOLOGY 64-65(2014):1-5.