Environmental connections of novel avian-origin H7N9 influenza virus infection and virus adaptation to the human

CORC > 天津工业生物技术研究所 > 蛋白质工程与疫苗实验室高福 > 期刊论文

	Environmental connections of novel avian-origin H7N9 influenza virus infection and virus adaptation to the human
	Li Jun 1; Yu XinFen 1; Pu XiaoYing 1; Xie Li 1; Sun YongXiang 2; Xiao HaiXia 5; Wang FenJuan 2; Din Hua 1; Wu Ying 3; Liu Di 3,6
刊名	SCIENCE CHINA-LIFE SCIENCES
	2013-06-01
卷号	56 期号:6 页码:485-492
关键词	H7N9 influenza A virus human adaptation epidemiology substitution
英文摘要	A novel H7N9 influenza A virus has been discovered as the causative identity of the emerging acute respiratory infection cases in Shanghai, China. This virus has also been identified in cases of infection in the neighboring area Hangzhou City in Zhejiang Province. In this study, epidemiologic, clinical, and virological data from three patients in Hangzhou who were confirmed to be infected by the novel H7N9 influenza A virus were collected and analyzed. Human respiratory specimens and chicken feces from a contacted free market were tested for influenza virus by real-time reverse transcription PCR (RT-PCR) and sequencing. The clinical features of the three cases were similar featured with high fever and severe respiratory symptoms; however, only one of the patients died. A certain degree of diversity was observed among the three Hangzhou viruses sequenced from human samples compared with other reported H7N9 influenza A viruses. The sequences of the novel avian-origin H7N9 influenza viruses from Hangzhou City contained important amino acid substitutions related to human adaptation. One of the Hangzhou viruses had gained a novel amino acid substitution (Q226I) in the receptor binding region of hemagglutinin. More importantly, the virus sequenced from the chicken feces had a 627E substitution in the PB2 protein instead of the mammalian-adapted 627K substitution that was found in the PB2 proteins from the Hangzhou viruses from the three patients. Therefore, the newly-emerging H7N9 virus might be under adaptation pressure that will help it "jump" from avian to human hosts. The significance of these substitutions needs further exploration, with both laboratory experiments and extensive field surveillance.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biology
研究领域[WOS]	Life Sciences & Biomedicine - Other Topics
关键词[WOS]	TRANSMISSION ; REPLICATION ; FERRETS ; MODEL ; PB2
收录类别	SCI
语种	英语
WOS记录号	WOS:000320035300001
内容类型	期刊论文
源URL	[http://124.16.173.210/handle/834782/1290]
专题	天津工业生物技术研究所_蛋白质工程与疫苗实验室高福_期刊论文
作者单位	1.Hangzhou Ctr Dis Control & Prevent, Hangzhou 310021, Zhejiang, Peoples R China 2.Xiaoshan Ctr Dis Control & Prevent, Hangzhou 311201, Zhejiang, Peoples R China 3.Chinese Acad Sci, Inst Microbiol, CAS Key Lab Pathogen Microbiol & Immunol, Beijing 100101, Peoples R China 4.Chinese Ctr Dis Control & Prevent, Natl Inst Viral Dis Control & Prevent, Beijing 102206, Peoples R China 5.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Lab Prot Engn & Vaccine, Tianjin 300308, Peoples R China 6.Chinese Acad Sci, Inst Microbiol, Network Informat Ctr, Beijing 100101, Peoples R China
推荐引用方式 GB/T 7714	Li Jun,Yu XinFen,Pu XiaoYing,et al. Environmental connections of novel avian-origin H7N9 influenza virus infection and virus adaptation to the human[J]. SCIENCE CHINA-LIFE SCIENCES,2013,56(6):485-492.
APA	Li Jun.,Yu XinFen.,Pu XiaoYing.,Xie Li.,Sun YongXiang.,...&Pan JingCao.(2013).Environmental connections of novel avian-origin H7N9 influenza virus infection and virus adaptation to the human.SCIENCE CHINA-LIFE SCIENCES,56(6),485-492.
MLA	Li Jun,et al."Environmental connections of novel avian-origin H7N9 influenza virus infection and virus adaptation to the human".SCIENCE CHINA-LIFE SCIENCES 56.6(2013):485-492.