Nanoparticle-enhanced generation of gene-transfected mesenchymal stem cells for in vivo cardiac repair

CORC > 上海硅酸盐研究所 > 中国科学院上海硅酸盐研究所 > 高性能陶瓷和超微结构国家重点实验室 > 期刊论文

	Nanoparticle-enhanced generation of gene-transfected mesenchymal stem cells for in vivo cardiac repair
	Zhu, Kai 1,2; Wu, Meiying 3; Lai, Hao 1,2; Guo, Changfa 1,2; Li, Jun 1,2; Wang, Yulin 1,2; Chen, Yu 3; Wang, Chunsheng 1,2; Shi, Jianlin 3
刊名	BIOMATERIALS
	2016
卷号	74 期号:0 页码:188-199
关键词	Large mesopore Mesoporous organosilica Gene transfection Stem-cell therapy Cardiac repair
英文摘要	Transplantation of gene-transfected bone marrow-derived mesenchymal stem cells (BMMSCs) is a promising strategy for ischemic myocardium repair, but current therapeutic strategy suffers from high toxicity and inefficient gene transfection in primary BMMSCs. Here we designed and synthesized molecularly organic-inorganic hybrid hollow mesoporous organosilica nanoparticles (HMONs) based on nano-synthetic chemistry, which are featured with concurrent large pore size over 20 nm, small particulate size, hollow cavity and high dispersity for gene transfection in BMMSCs and subsequent in vivo cardiac repair. To efficiently create the therapeutic gene-transfected stem-cell lines, hepatocyte growth factor (HGF) gene was applied to transfect BMMSCs via biocompatible surface-engineered HMONs as a high-performance gene-delivery nanosystem. On the rat model of myocardial infarction, transplantation of HGF gene-transfected BMMSCs enables the largely decreased apoptotic cardiomyocytes, reduced infarct scar size, relieved interstitial fibrosis, and increased angiogenesis in myocardium. The resultant cardiac repair further promotes the significant improvement of heart function. Therefore, the fabricated organic inorganic hybrid HMONs with large pore size represent a generalizable strategy and platform for gene transfection in BMMSCs and further regenerative medicine. (C) 2015 Elsevier Ltd. All rights reserved.
WOS标题词	Science & Technology ; Technology
类目[WOS]	Engineering, Biomedical ; Materials Science, Biomaterials
研究领域[WOS]	Engineering ; Materials Science
关键词[WOS]	MESOPOROUS SILICA NANOPARTICLES ; HEPATOCYTE GROWTH-FACTOR ; MYOCARDIAL-INFARCTION ; DRUG-DELIVERY ; BIOMEDICAL APPLICATIONS ; PHASE-I ; DISEASE ; THERAPY ; DESIGN ; SIRNA
收录类别	SCI
语种	英语
WOS记录号	WOS:000364610800017
内容类型	期刊论文
源URL	[http://ir.sic.ac.cn/handle/331005/8217]
专题	上海硅酸盐研究所_高性能陶瓷和超微结构国家重点实验室_期刊论文
作者单位	1.Fudan Univ, Zhongshan Hosp, Dept Cardiac Surg, Shanghai 200032, Peoples R China 2.Shanghai Inst Cardiovasc Dis, Shanghai 200032, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Ceram, State Key Lab High Performance Ceram & Superfine, Shanghai 200050, Peoples R China
推荐引用方式 GB/T 7714	Zhu, Kai,Wu, Meiying,Lai, Hao,et al. Nanoparticle-enhanced generation of gene-transfected mesenchymal stem cells for in vivo cardiac repair[J]. BIOMATERIALS,2016,74(0):188-199.
APA	Zhu, Kai.,Wu, Meiying.,Lai, Hao.,Guo, Changfa.,Li, Jun.,...&Shi, Jianlin.(2016).Nanoparticle-enhanced generation of gene-transfected mesenchymal stem cells for in vivo cardiac repair.BIOMATERIALS,74(0),188-199.
MLA	Zhu, Kai,et al."Nanoparticle-enhanced generation of gene-transfected mesenchymal stem cells for in vivo cardiac repair".BIOMATERIALS 74.0(2016):188-199.