Immunomodulatory Activity of Polysaccharide-Protein Complex from the Mushroom Sclerotia of Polyporus rhinocerus in Murine Macrophages

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	Immunomodulatory Activity of Polysaccharide-Protein Complex from the Mushroom Sclerotia of Polyporus rhinocerus in Murine Macrophages
	Liu, Chaoran 1; Chen, Jialun 1; Chen, Lei 2; Huang, Xuesong 3; Cheung, Peter C. K.1
刊名	JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
	2016-04-27
卷号	64 期号:16 页码:3206-3214
关键词	beta-mannoglucans macrophage activation mushroom sclerotia polysaccharide-protein complex Polyporus rhinocerus
英文摘要	A novel water-soluble polysaccharide-protein complex (PRW1) isolated from the sclerotia of an edible mushroom Polyporus rhinocerus which was purified by membrane ultrafiltration could significantly activate murine macrophages RAW264.7 in vitro. PRW1 had a molecular weight of less than SO kDa and was found to be a highly branched heteropolysaccharide-protein complex composed of 45.7 +/- 0.97% polysaccharide and 44.2 +/- 0.41% protein. Based on the results of total acid hydrolysis, methylation analysis, and Fourier transform infrared spectroscopy, the carbohydrate moiety of PRW1 was found to be a beta-D-mannoglucan with its backbone containing -> 1)-D-Glcp-(4 ->, -> 1)-D-Glcp-(6 ->, and -> 1)-D-Manp-(2 -> residues (molar ratio of 5:4:6) and having terminal D-Glcp as side chain (degree of branching of 0.62). In vitro studies showed that PRW1 significantly induced NO production and enhanced the release of a variety of cytokines including G-CSF, GM-CSF, IL-6, IL12p40/70, MCP-1, MCP-5, MIP-1-alpha, MIP-2, RANTES, sTNFRI, and TNF-alpha. Mechanistically, PRW1 treatment triggered ERK phosphorylation to activate macrophages within 15 min and significantly increased the expression level of inducible NOS after 6 h. In summary, this study indicates that PRW1 derived from the sclerotia of P. rhinocerus is a potential immunomodulatory agent for cancer immunotherapy.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]	Agriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology
研究领域[WOS]	Agriculture ; Chemistry ; Food Science & Technology
关键词[WOS]	DIFFERENT DEVELOPMENTAL-STAGES ; PLEUROTUS-TUBER-REGIUM ; HOT-WATER EXTRACT ; LYCIUM-BARBARUM L ; COOKE RYVARDEN ; MILK MUSHROOM ; BETA-GLUCAN ; CELL ; ACTIVATION ; IMMUNE
收录类别	SCI
语种	英语
WOS记录号	WOS:000375244300012
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn/handle/337004/8285]
专题	青岛生物能源与过程研究所_多相催化转化团队
作者单位	1.Chinese Univ Hong Kong, Sch Life Sci, Food & Nutr Sci, Shatin, Hong Kong, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao Engn Res Ctr Biomass Resources & Environm, Key Lab Biofuels, Qingdao 266101, Peoples R China 3.Jinan Univ, Dept Food Sci & Engn, Guangzhou 510632, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Liu, Chaoran,Chen, Jialun,Chen, Lei,et al. Immunomodulatory Activity of Polysaccharide-Protein Complex from the Mushroom Sclerotia of Polyporus rhinocerus in Murine Macrophages[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2016,64(16):3206-3214.
APA	Liu, Chaoran,Chen, Jialun,Chen, Lei,Huang, Xuesong,&Cheung, Peter C. K..(2016).Immunomodulatory Activity of Polysaccharide-Protein Complex from the Mushroom Sclerotia of Polyporus rhinocerus in Murine Macrophages.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,64(16),3206-3214.
MLA	Liu, Chaoran,et al."Immunomodulatory Activity of Polysaccharide-Protein Complex from the Mushroom Sclerotia of Polyporus rhinocerus in Murine Macrophages".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 64.16(2016):3206-3214.