Development of a 3-hydroxypropionate resistant Escherichia coli strain

CORC > 青岛生物能源与过程研究所 > 中国科学院青岛生物能源与过程研究所 > 材料生物技术研究中心

	Development of a 3-hydroxypropionate resistant Escherichia coli strain
	Liu, Min 1,2; Han, Xueping 1,2; Xian, Mo 1; Ding, Yamei 3; Liu, Huizhou 1; Zhao, Guang 1
刊名	BIOENGINEERED
	2016
卷号	7 期号:1 页码:21-27
关键词	bacterial stress response comparative proteomics Escherichia coli 3-hydroxypropionate 3-hydroxypropionate resistance
英文摘要	3-hydroxypropionate (3HP) is an important platform chemical, and its biosynthesis is severely restricted by the toxicity of 3HP on cell growth and survival. To improve Escherichia coli resistance to 3HP and reduce the total production cost in industrial applications, we have identified variations in protein expression level exposed to sub-lethal concentration of this chemical using 2-dimensional gel electrophoresis. Under 3HP stress, the amount of 46 proteins was increased while the amount of 23 proteins was reduced. According to the proteomic results, overexpression of some identified proteins significantly increased the E. coli survival rate under 3HP stress. This study shed light on clues for developing E. coli strains with higher resistance to 3HP toxicity and lower production cost for industrial applications.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology
研究领域[WOS]	Biotechnology & Applied Microbiology
关键词[WOS]	PROLINE BIOSYNTHESIS ; PROTEOME ANALYSIS ; ACID RESISTANCE ; STRESS ; IDENTIFICATION ; DEHYDROGENASE ; MECHANISMS ; TOLERANCE ; SURVIVAL ; GLYCEROL
收录类别	SCI
语种	英语
WOS记录号	WOS:000373159100009
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn/handle/337004/8246]
专题	青岛生物能源与过程研究所_材料生物技术研究中心
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biobased Mat, Qingdao, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Liu, Min,Han, Xueping,Xian, Mo,et al. Development of a 3-hydroxypropionate resistant Escherichia coli strain[J]. BIOENGINEERED,2016,7(1):21-27.
APA	Liu, Min,Han, Xueping,Xian, Mo,Ding, Yamei,Liu, Huizhou,&Zhao, Guang.(2016).Development of a 3-hydroxypropionate resistant Escherichia coli strain.BIOENGINEERED,7(1),21-27.
MLA	Liu, Min,et al."Development of a 3-hydroxypropionate resistant Escherichia coli strain".BIOENGINEERED 7.1(2016):21-27.