Identification and cloning of class II and III chitinases from alkaline floral nectar of Rhododendron irroratum, Ericaceae

CORC > 昆明植物研究所 > 中国科学院昆明植物研究所 > 中国科学院东亚植物多样性与生物地理学重点实验室

	Identification and cloning of class II and III chitinases from alkaline floral nectar of Rhododendron irroratum, Ericaceae
	Zha, Hong-Guang; Milne, Richard I.; Zhou, Hong-Xia; Chen, Xiang-Yang; Sun, Hang
刊名	PLANTA
	2016-10-01
卷号	244 期号:4 页码:805-818
关键词	Alkaline nectar Chitinolytic activity Class II chitinase Class III chitinase Glycoside hydrolase Pathogenesis-related proteins
英文摘要	Previous studies have demonstrated antimicrobial activity in plant floral nectar, but the molecular basis for the mechanism is still poorly understood. Two chitinases, class II (Rhchi2) and III (Rhchi3), were characterized from alkaline Rhododendron irroratum nectar by both SDS-PAGE and mass spectrometry. Rhchi2 (27 kDa) and Rhchi3 (29 kDa) are glycoside hydrolases (family 19 and 18) with theoretical pI of 8.19 and 7.04. The expression patterns of Rhchi2 and Rhchi3 were analyzed by semi-quantitative RT-PCR. Rhchi2 is expressed in flowers (corolla nectar pouches) and leaves while Rhchi3 is expressed in flowers. Chitinase in concentrated protein and fresh nectar samples was visualised by SDS-PAGE and chitinolytic activity in fresh nectar was determined spectrophotometrically via chitin-azure. Full length gene sequences were cloned with Tail-PCR and RACE. The amino acid sequence deduced from the coding region for these proteins showed high identity with known chitinases and predicted to be located in extracellular space. Fresh R. irroratum floral nectar showed significant chitinolytic activity. Our results demonstrate that class III chitinase (GH 18 family) also exists in floral nectar. The functional relationship between class II and III chitinases and the role of these pathogenesis-related proteins in antimicrobial activity in nectar is suggested.
类目[WOS]	Plant Sciences
研究领域[WOS]	Plant Sciences
关键词[WOS]	POLYACRYLAMIDE-GEL ELECTROPHORESIS ; PLANT CHITINASES ; EXTRAFLORAL NECTAR ; SUBCELLULAR-LOCALIZATION ; HYDROGEN-PEROXIDE ; HETEROLOGOUS EXPRESSION ; GLYCOSIDE HYDROLASES ; PHYSIOLOGICAL ROLES ; TRANSGENIC TOBACCO ; GENE-EXPRESSION
收录类别	SCI
语种	英语
WOS记录号	WOS:000382939900004
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn/handle/151853/28636]
专题	昆明植物研究所_中国科学院东亚植物多样性与生物地理学重点实验室
推荐引用方式 GB/T 7714	Zha, Hong-Guang,Milne, Richard I.,Zhou, Hong-Xia,et al. Identification and cloning of class II and III chitinases from alkaline floral nectar of Rhododendron irroratum, Ericaceae[J]. PLANTA,2016,244(4):805-818.
APA	Zha, Hong-Guang,Milne, Richard I.,Zhou, Hong-Xia,Chen, Xiang-Yang,&Sun, Hang.(2016).Identification and cloning of class II and III chitinases from alkaline floral nectar of Rhododendron irroratum, Ericaceae.PLANTA,244(4),805-818.
MLA	Zha, Hong-Guang,et al."Identification and cloning of class II and III chitinases from alkaline floral nectar of Rhododendron irroratum, Ericaceae".PLANTA 244.4(2016):805-818.