A Geranylfarnesyl Diphosphate Synthase Provides the Precursor for Sesterterpenoid (C-25) Formation in the Glandular Trichomes of the Mint Species Leucosceptrum canum

CORC > 昆明植物研究所 > 中国科学院昆明植物研究所 > 植物化学与西部植物资源持续利用国家重点实验室

	A Geranylfarnesyl Diphosphate Synthase Provides the Precursor for Sesterterpenoid (C-25) Formation in the Glandular Trichomes of the Mint Species Leucosceptrum canum
	Liu, Yan 1; Luo, Shi-Hong 1; Schmidt, Axel 2; Wang, Guo-Dong 3,4; Sun, Gui-Ling5 ; Grant, Marcus 2; Kuang, Ce 1; Yang, Min-Jie 1; Jing, Shu-Xi 1; Li, Chun-Huan 1
刊名	PLANT CELL
	2016-03-01
卷号	28 期号:3 页码:804-822
英文摘要	Plant sesterterpenoids, an important class of terpenoids, are widely distributed in various plants, including food crops. However, little is known about their biosynthesis. Here, we cloned and functionally characterized a plant geranylfarnesyl diphosphate synthase (Lc-GFDPS), the enzyme producing the C-25 prenyl diphosphate precursor to all sesterterpenoids, from the glandular trichomes of the woody plant Leucosceptrum canum. GFDPS catalyzed the formation of GFDP after expression in Escherichia coli. Overexpressing GFDPS in Arabidopsis thaliana also gave an extract catalyzing GFDP formation. GFDPS was strongly expressed in glandular trichomes, and its transcript profile was completely in accordance with the sesterterpenoid accumulation pattern. GFDPS is localized to the plastids, and inhibitor studies indicated its use of isoprenyl diphosphate substrates supplied by the 2-C-methyl-D-erythritol 4-phosphate pathway. Application of a jasmonate defense hormone induced GFDPS transcript and sesterterpenoid accumulation, while reducing feeding and growth of the generalist insect Spodoptera exigua, suggesting that these C-25 terpenoids play a defensive role. Phylogenetic analysis suggested that GFDPS probably evolved from plant geranylgeranyl diphosphate synthase under the influence of positive selection. The isolation of GFDPS provides a model for investigating sesterterpenoid formation in other species and a tool for manipulating the formation of this group in plants and other organisms.
类目[WOS]	Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology
研究领域[WOS]	Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology
关键词[WOS]	HORIZONTAL GENE-TRANSFER ; GENOME MINING APPROACH ; SPRUCE PICEA-ABIES ; ISOPRENOID BIOSYNTHESIS ; ARABIDOPSIS-THALIANA ; GERANYL DIPHOSPHATE ; DEFENSIVE SESTERTERPENOIDS ; TERPENOID BIOSYNTHESIS ; SALVIA YOSGADENSIS ; ACID BIOSYNTHESIS
收录类别	SCI
语种	英语
WOS记录号	WOS:000375415100018
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn/handle/151853/26225]
专题	昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Peoples R China 2.Max Planck Inst Chem Ecol, D-07745 Jena, Germany 3.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Genom, Beijing 100101, Peoples R China 4.Chinese Acad Sci, Natl Ctr Plant Gene Res, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 5.Chinese Acad Sci, Kunming Inst Bot, Key Lab Econ Plants & Biotechnol, Kunming 650201, Peoples R China
推荐引用方式 GB/T 7714	Liu, Yan,Luo, Shi-Hong,Schmidt, Axel,et al. A Geranylfarnesyl Diphosphate Synthase Provides the Precursor for Sesterterpenoid (C-25) Formation in the Glandular Trichomes of the Mint Species Leucosceptrum canum[J]. PLANT CELL,2016,28(3):804-822.
APA	Liu, Yan.,Luo, Shi-Hong.,Schmidt, Axel.,Wang, Guo-Dong.,Sun, Gui-Ling.,...&Li, Sheng-Hong.(2016).A Geranylfarnesyl Diphosphate Synthase Provides the Precursor for Sesterterpenoid (C-25) Formation in the Glandular Trichomes of the Mint Species Leucosceptrum canum.PLANT CELL,28(3),804-822.
MLA	Liu, Yan,et al."A Geranylfarnesyl Diphosphate Synthase Provides the Precursor for Sesterterpenoid (C-25) Formation in the Glandular Trichomes of the Mint Species Leucosceptrum canum".PLANT CELL 28.3(2016):804-822.