Detection of p53 gene mutation by using a novel biosensor based on localized surface plasmon resonance

CORC > 光电技术研究所 > 中国科学院光电技术研究所 > 微细加工光学技术国家重点实验室（开放室）

	Detection of p53 gene mutation by using a novel biosensor based on localized surface plasmon resonance
	Duan, R. Q.; Yuan, J. L.; Yang, H.; Luo, X. G.; Xi, M. R.
刊名	NEOPLASMA
	2012
卷号	59 期号:3 页码:348-353
ISSN号	0028-2685
通讯作者	Xi, MR (reprint author), Sichuan Univ, Dept Gynecol & Obstet, W China Univ Hosp 2, Chengdu 610041, Peoples R China.
中文摘要	Few studies to date have reported on the development and application of a nanobiosensor based on localized surface plasmon resonance (LSPR) for detecting gene mutations. This study aimed to develop a novel LSPR biosensor used for detecting p53 mutation. Nanosphere lithography was used to fabricate the silver nanoparticles. The DNA probe was designed to recognize the target sequence and immobilized on the chip surface by a covalent-coupling method using amine-group ligands. Synthetic oligonucleotides or PCR products were amplified from genomic DNA taken from blood samples and hybridized with the immobilized probe. Wild-type and mutant p53 was detected by measuring shifts in peak of LSPR extinction spectra. The low detection limit of the sensor for target sequence was 10 nM, and detection occurred over a wide dynamic range (10 nM - 10 mu M). Importantly, the differences in measuring signal between wild-type and mismatched p53 DNA was significant, allowing for this sensor to effectively discriminate against single base mutations. In conclusion, we developed a biosensor with potential as a rapid, label-free, sensitive, and low-cost method for detecting p53 mutation. Our results suggest that such an LSPR-based biosensor provides an attractive alternative for clinical detection of genetic mutation.
英文摘要	Few studies to date have reported on the development and application of a nanobiosensor based on localized surface plasmon resonance (LSPR) for detecting gene mutations. This study aimed to develop a novel LSPR biosensor used for detecting p53 mutation. Nanosphere lithography was used to fabricate the silver nanoparticles. The DNA probe was designed to recognize the target sequence and immobilized on the chip surface by a covalent-coupling method using amine-group ligands. Synthetic oligonucleotides or PCR products were amplified from genomic DNA taken from blood samples and hybridized with the immobilized probe. Wild-type and mutant p53 was detected by measuring shifts in peak of LSPR extinction spectra. The low detection limit of the sensor for target sequence was 10 nM, and detection occurred over a wide dynamic range (10 nM - 10 mu M). Importantly, the differences in measuring signal between wild-type and mismatched p53 DNA was significant, allowing for this sensor to effectively discriminate against single base mutations. In conclusion, we developed a biosensor with potential as a rapid, label-free, sensitive, and low-cost method for detecting p53 mutation. Our results suggest that such an LSPR-based biosensor provides an attractive alternative for clinical detection of genetic mutation.
学科主题	biosensor; localized surface plasm on resonance; gene mutation
收录类别	SCI
语种	英语
WOS记录号	WOS:000301966700015
内容类型	期刊论文
源URL	[http://ir.ioe.ac.cn/handle/181551/6809]
专题	光电技术研究所_微细加工光学技术国家重点实验室（开放室）
作者单位	1.[Duan, R. Q. 2.Yuan, J. L. 3.Xi, M. R.] Sichuan Univ, Dept Gynecol & Obstet, W China Univ Hosp 2, Chengdu 610041, Peoples R China 4.[Yang, H. 5.Luo, X. G.] Chinese Acad Sci, State Key Lab Opt Technol Microfabricat, Inst Opt & Elect, Chengdu 610209, Peoples R China
推荐引用方式 GB/T 7714	Duan, R. Q.,Yuan, J. L.,Yang, H.,et al. Detection of p53 gene mutation by using a novel biosensor based on localized surface plasmon resonance[J]. NEOPLASMA,2012,59(3):348-353.
APA	Duan, R. Q.,Yuan, J. L.,Yang, H.,Luo, X. G.,&Xi, M. R..(2012).Detection of p53 gene mutation by using a novel biosensor based on localized surface plasmon resonance.NEOPLASMA,59(3),348-353.
MLA	Duan, R. Q.,et al."Detection of p53 gene mutation by using a novel biosensor based on localized surface plasmon resonance".NEOPLASMA 59.3(2012):348-353.