Molecular separation of two long taxonomically debated cyanobacterial genera Cylindrospermopsis and Raphidiopsis (Nostocales) based on the ITS-L phylogeny.

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	Molecular separation of two long taxonomically debated cyanobacterial genera Cylindrospermopsis and Raphidiopsis (Nostocales) based on the ITS-L phylogeny.
	Li, Xiaochuang 1,2; Li, Shouchun 3; Kong, Renqiu 1; Li, Renhui 1
刊名	HARMFUL ALGAE
	2016-07-01
卷号	57 页码:88-97
关键词	Cylindrospermopsis ITS-L Phylogeny Raphidiopsis Taxonomy
ISSN号	1568-9883
英文摘要	The cyanobacterial genera Raphidiopsis and Cylindrospermopsis morphologically resembled each other and are difficult to distinguish, especially when heterocytes do not form in Cylindrospermopsis species. The phylogeny based on multiple gene loci sequences in previous studies cannot divide the strains of these two genera into separate clusters. In the present study, four gene loci 16S rRNA, ITS-L, cpcBA-IGS and rpoC1, sequenced from many Chinese strains (44 Cylindrospermopsis strains and 16 Raphidiopsis strains), were analyzed to infer the phylogenetic relationship between the two genera. According to the 16S rRNA, cpcBA-IGS and rpoC1 gene phylogeny, Cylindrospermopsis and Raphidiopsis strains are intermixed and divide into two or three groups. By contrast, the ITS-L analysis implied that they respectively form two separate and unique clusters. A fragment of 7 bp (RAGAAACT) in ITS-L was conserved for Raphidiopsis strains, which could be distinguished from Cylindrospermopsis strains. A pair of primers targeting the ITS-L fragment specific to Raphidiopsis strains were designed and further verified. (C) 2016 Elsevier B.V. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Marine & Freshwater Biology
研究领域[WOS]	Marine & Freshwater Biology
关键词[WOS]	POPULATION-DYNAMICS ; SUBBA RAJU ; MEDITERRANEA SKUJA ; FLORIDA LAKES ; 1ST REPORT ; RACIBORSKII ; RESERVOIRS ; DOMINANCE ; SEQUENCES ; RELEASE
收录类别	SCI
语种	英语
WOS记录号	WOS:000381544600009
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/28345]
专题	水生生物研究所_藻类生物学及应用研究中心_学位论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Jiangxi Normal Univ, Coll Life Sci, Nanchang 330027, Peoples R China
推荐引用方式 GB/T 7714	Li, Xiaochuang,Li, Shouchun,Kong, Renqiu,et al. Molecular separation of two long taxonomically debated cyanobacterial genera Cylindrospermopsis and Raphidiopsis (Nostocales) based on the ITS-L phylogeny.[J]. HARMFUL ALGAE,2016,57:88-97.
APA	Li, Xiaochuang,Li, Shouchun,Kong, Renqiu,&Li, Renhui.(2016).Molecular separation of two long taxonomically debated cyanobacterial genera Cylindrospermopsis and Raphidiopsis (Nostocales) based on the ITS-L phylogeny..HARMFUL ALGAE,57,88-97.
MLA	Li, Xiaochuang,et al."Molecular separation of two long taxonomically debated cyanobacterial genera Cylindrospermopsis and Raphidiopsis (Nostocales) based on the ITS-L phylogeny.".HARMFUL ALGAE 57(2016):88-97.