Immunogenicity and protective role of antigenic regions from five outer membrane proteins of Flavobacterium columnare in grass carp Ctenopharyngodon idella

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	Immunogenicity and protective role of antigenic regions from five outer membrane proteins of Flavobacterium columnare in grass carp Ctenopharyngodon idella
	Luo Zhang 1,2,3; Liu Zhixin 2; Fu Jianping 2; Zhang Qiusheng 2; Huang Bei 4; Nie Pin 2
刊名	CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
	2016-11-01
卷号	34 期号:6 页码:1247-1257
关键词	Flavobacterium columnare outer membrane protein antigen immunogenicity vaccine immune response grass carp
ISSN号	0254-4059
英文摘要	Flavobacterium columnare causes columnaris disease in freshwater fish. In the present study, the antigenic regions of five outer membrane proteins (OMPs), including zinc metalloprotease, prolyl oligopeptidase, thermolysin, collagenase and chondroitin AC lyase, were bioinformatically analyzed, fused together, and then expressed as a recombinant fusion protein in Escherichia coli. The expressed protein of 95.6 kDa, as estimated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis, was consistent with the molecular weight deduced from the amino acid sequence. The purified recombinant protein was used to vaccinate the grass carp, Ctenopharyngodon idella. Following vaccination of the fish their IgM antibody levels were examined, as was the expression of IgM, IgD and IgZ immunoglobulin genes and other genes such as MHC I alpha and MHC II beta, which are also involved in adaptive immunity. Interleukin genes (IL), including IL-1 beta, IL-8 and IL-10, and type I and type II interferon (IFN) genes were also examined. At 3 and 4 weeks post-vaccination (wpv), significant increases in IgM antibody levels were observed in the fish vaccinated with the recombinant fusion protein, and an increase in the expression levels of IgM, IgD and IgZ genes was also detected following the vaccinations, thus indicating that an adaptive immune response was induced by the vaccinations. Early increases in the expression levels of IL and IFN genes were also observed in the vaccinated fish. At four wpv, the fish were challenged with F. columnare, and the vaccinated fish showed a good level of protection against this pathogen, with 39% relative percent survival (RPS) compared with the control group. It can be concluded, therefore, that the five OMPs, in the form of a recombinant fusion protein vaccine, induced an immune response in fish and protection against F. columnare.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]	Limnology ; Oceanography
研究领域[WOS]	Marine & Freshwater Biology ; Oceanography
关键词[WOS]	TILAPIA OREOCHROMIS-NILOTICUS ; RAINBOW-TROUT ; IMMUNE-RESPONSE ; AEROMONAS-HYDROPHILA ; VIBRIO-ANGUILLARUM ; VACCINE CANDIDATE ; CHANNEL CATFISH ; FISH ; EXPRESSION ; IDENTIFICATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000382885200010
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/28368]
专题	水生生物研究所_淡水生态学研究中心_学位论文
作者单位	1.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 3.Tianjin Fishery Res Inst, Tianjin 300221, Peoples R China 4.Jimei Univ, Coll Fisheries, Xiamen 361021, Peoples R China
推荐引用方式 GB/T 7714	Luo Zhang,Liu Zhixin,Fu Jianping,et al. Immunogenicity and protective role of antigenic regions from five outer membrane proteins of Flavobacterium columnare in grass carp Ctenopharyngodon idella[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2016,34(6):1247-1257.
APA	Luo Zhang,Liu Zhixin,Fu Jianping,Zhang Qiusheng,Huang Bei,&Nie Pin.(2016).Immunogenicity and protective role of antigenic regions from five outer membrane proteins of Flavobacterium columnare in grass carp Ctenopharyngodon idella.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,34(6),1247-1257.
MLA	Luo Zhang,et al."Immunogenicity and protective role of antigenic regions from five outer membrane proteins of Flavobacterium columnare in grass carp Ctenopharyngodon idella".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 34.6(2016):1247-1257.