Molecular characterization and expression pattern of a germ cell marker gene dnd in gibel carp (Carassius gibelio)

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	Molecular characterization and expression pattern of a germ cell marker gene dnd in gibel carp (Carassius gibelio)
	Li, Shi-Zhu 1; Liu, Wei 2; Li, Zhi 1; Wang, Yang 1; Zhou, Li 1; Yi, Mei-Sheng 2; Gui, Jian-Fang 1
刊名	GENE
	2016-10-10
卷号	591 期号:1 页码:183-190
关键词	Dead end Primordial germ cell Germ cells Oogenesis Spermatogenesis
ISSN号	0378-1119
英文摘要	As a germ cell marker gene, Dead end (dnd) has been identified and characterized in many vertebrates. Recently, we created a complete germ cell-depleted gonad model by the dnd-specific morpholino-mediated knockdown approach, and revealed sex-biased gene expression alteration through utilizing unisexual gynogenetic superiority in polyploid gibel carp. However, dud and its expression pattern are still unclear in the gibel carp. In this study, we further analyzed molecular characterization of gibel carp dnd and its dynamic expression pattern during gametogenesis and embryogenesis. Similar to other homologs in vertebrates, gibel carp dnd contains a conserved RRM motif and five other motifs, and is highly evolutionary conserved in genomic organization and neighborhood gene synteny. RT-PCR and Western blot analyses showed its gonad-specific expression intensively in testis and ovary. Section in situ hybridization (SISH) and immunofluorescence localization revealed its dynamic expression pattern specific to oogenic cells and spermatogenetic cells during oogenesis and spermatogenesis. Moreover, its temporal and spatial distribution specific to PGCs were also demonstrated by RT-PCR and whole mount in situ hybridization (WISH) during embryogenesis. Therefore, gibel carp Dnd is a conserved germ cell marker during gametogenesis, and its maternal transcript is also a useful marker for tracing PGC specification and migration. (C) 2016 Elsevier B.V. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Genetics & Heredity
研究领域[WOS]	Genetics & Heredity
关键词[WOS]	DEAD-END GENE ; AURATUS-GIBELIO ; REPRODUCTION MODES ; CORTICAL GRANULES ; ZEBRAFISH EMBRYOS ; SEXUAL-DIMORPHISM ; BINDING-PROTEIN ; MESSENGER-RNA ; FISH ; OOCYTES
收录类别	SCI
语种	英语
WOS记录号	WOS:000382409400026
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/28348]
专题	水生生物研究所_藻类生物学及应用研究中心_学位论文
作者单位	1.Chinese Acad Sci, Grad Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Sun Yat Sen Univ, Sch Marine Sci, Zhuhai Key Lab Marine Bioresources & Environm, Guangzhou, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Li, Shi-Zhu,Liu, Wei,Li, Zhi,et al. Molecular characterization and expression pattern of a germ cell marker gene dnd in gibel carp (Carassius gibelio)[J]. GENE,2016,591(1):183-190.
APA	Li, Shi-Zhu.,Liu, Wei.,Li, Zhi.,Wang, Yang.,Zhou, Li.,...&Gui, Jian-Fang.(2016).Molecular characterization and expression pattern of a germ cell marker gene dnd in gibel carp (Carassius gibelio).GENE,591(1),183-190.
MLA	Li, Shi-Zhu,et al."Molecular characterization and expression pattern of a germ cell marker gene dnd in gibel carp (Carassius gibelio)".GENE 591.1(2016):183-190.