Cdk3, a conjugation-specific cyclin-dependent kinase, is essential for the initiation of meiosis in Tetrahymena thermophila

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	Cdk3, a conjugation-specific cyclin-dependent kinase, is essential for the initiation of meiosis in Tetrahymena thermophila
	Yan, Guan-xiong 1,2; Zhang, Jing 1,2; Shodhan, Anura 3; Tian, Miao 1,3,4; Miao, Wei 1
刊名	CELL CYCLE
	2016
卷号	15 期号:18 页码:2506-2514
关键词	conjugation cyclin-dependent kinase meiosis initiation sexual reproduction Tetrahymena thermophila
ISSN号	1538-4101
英文摘要	Meiosis is an important process in sexual reproduction. Meiosis initiation has been found to be highly diverse among species. In yeast, it has been established that cyclin-dependent kinases (Cdks) and cyclins are essential components in the meiosis initiation pathway. In this study, we identified 4 Cdks in the model ciliate, Tetrahymena thermophila, and we found one of them, Cdk3, which is specifically expressed during early conjugation, to be essential for meiosis initiation. Cdk3 deletion led to arrest at the pair formation stage of conjugation. We then confirmed that Cdk3 acts upstream of double-strand break (DSB) formation. Moreover, we detected that Cdk3 is necessary for the expression of many genes involved in early meiotic events. Through proteomic quantification of phosphorylation, co-expression analysis and RNA-Seq analyses, we identified a conjugation-specific cyclin, Cyc2, which most likely partners with Cdk3 to initiate meiosis.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Cell Biology
研究领域[WOS]	Cell Biology
关键词[WOS]	CELL-CYCLE ; DNA-REPLICATION ; PROTEIN-KINASE ; GENE ONTOLOGY ; FISSION YEAST ; RNA-SEQ ; PHOSPHORYLATION ; IME2 ; ACTIVATION ; GERMLINE
收录类别	SCI
语种	英语
WOS记录号	WOS:000384022200021
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/28357]
专题	水生生物研究所_水生生物多样性与资源保护研究中心_学位论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Univ Vienna, Dept Chromosome Biol, Ctr Mol Biol, Vienna, Austria 4.Univ Vienna, Max F Perutz Labs, Ctr Mol Biol, Vienna, Austria
推荐引用方式 GB/T 7714	Yan, Guan-xiong,Zhang, Jing,Shodhan, Anura,et al. Cdk3, a conjugation-specific cyclin-dependent kinase, is essential for the initiation of meiosis in Tetrahymena thermophila[J]. CELL CYCLE,2016,15(18):2506-2514.
APA	Yan, Guan-xiong,Zhang, Jing,Shodhan, Anura,Tian, Miao,&Miao, Wei.(2016).Cdk3, a conjugation-specific cyclin-dependent kinase, is essential for the initiation of meiosis in Tetrahymena thermophila.CELL CYCLE,15(18),2506-2514.
MLA	Yan, Guan-xiong,et al."Cdk3, a conjugation-specific cyclin-dependent kinase, is essential for the initiation of meiosis in Tetrahymena thermophila".CELL CYCLE 15.18(2016):2506-2514.