Dietary low or excess levels of lipids reduced growth performance, and impaired immune function and structure of head kidney, spleen and skin in young grass carp (Ctenopharyngodon idella) under the infection of Aeromonas hydrophila

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	Dietary low or excess levels of lipids reduced growth performance, and impaired immune function and structure of head kidney, spleen and skin in young grass carp (Ctenopharyngodon idella) under the infection of Aeromonas hydrophila
	Ni, Pei-Jun 1; Jiang, Wei-Dan 1,2,3; Wu, Pei 1,2,3; Liu, Yang 1,2,3; Kuang, Sheng-Yao 4; Tang, Ling 4; Tang, Wu-Neng 4; Zhang, Yong-An 5; Zhou, Xiao-Qiu 1,2,3; Feng, Lin 1,2,3
刊名	FISH & SHELLFISH IMMUNOLOGY
	2016-08-01
卷号	55 页码:28-47
关键词	Ctenopharyngodon idella Lipids Immune organs Bacterial challenge Structure integrity Immune function
ISSN号	1050-4648
英文摘要	Our study explored the effect of dietary lipids on growth and immunity and structure (head kidney, spleen and skin) of young grass, carp (Ctenopharyngodon idella). A total of 540 young grass carp with an average initial weight of 261.41 +/- 0.53 g were fed diets containing six graded levels of lipids at 5.9 -80.1 WIT diet for 8 weeks. After that, a challenge trial was conducted by injection of Aeromonas hydrophila over 2 weeks. The results indicated that compared with optimal lipids supplementation, low and excess levels of lipids down-regulated the mRNA levels of antimicrobial peptides, anti-inflammatory cytokines, inhibitor of kappa B alpha (I kappa B alpha) and ribosomal p70S6 kinase (S6K1), and up-regulated pro-inflammatory cytokines, nuclear factor kappa B p65 (NF-kappa B p65), NF-kappa B c-Rel (not p52), I kappa B kinase alpha (IKK alpha), IKK beta, IKK gamma, and eIF4E-binding protein (4EBP) mRNA levels in the head kidney and spleen of young grass carp (P < 0.05). Low or excess levels of lipids also increased reactive oxygen species (ROS) production and malondialdehyde (MDA) and protein carbonyl (PC) contents, reduced the activities of antioxidant enzymes (P < 0.05), down-regulate the relative mRNA levels of antioxidant enzymes and NF-E2-related factor 2 (Nrf2), and up-regulated the expression levels of Kelch-like ECH-associating protein la (Keap1a) and Keap1b in the head kidney and spleen. In addition, low or excess levels of lipids down regulated the mRNA levels of B-cell lymphoma-2 (Bcl-2) and inhibitor of apoptosis protein (IAP) in the head kidney and spleen, whereas up-regulated the mRNA levels of apoptotic protease activating factor-1 (Apaf-1), caspase 3, 7, 8 and 9 mRNA levels in the head kidney and spleen and Fas ligand (FasL) mRNA levels in the spleen of young grass carp, suggesting that low or excess levels of lipids could decrease the head kidney and spleen immune function, induce oxidative damage and apoptosis and impair antioxidant system of young grass carp. At last, low or excess levels of lipids also impaired the immune function and structure in the skin of young grass carp. Based on the quadratic regression analysis for PWG, skin haemorrhage and lesions morbidity and IgM content, the dietary lipids requirements for young grass carp were estimated to be 43.7, 60.2, 55.0 and 52.1 g/kg diet, respectively. (C) 2016 Published by Elsevier Ltd.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
研究领域[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
关键词[WOS]	NF-KAPPA-B ; TIGHT JUNCTION PROTEINS ; SALMON SALMO-SALAR ; ANTIOXIDANT ENZYME-ACTIVITIES ; SOLEA-SENEGALENSIS JUVENILES ; INTESTINAL MUCOSAL IMMUNE ; MESSENGER-RNA EXPRESSION ; OXIDATIVE STRESS ; CYPRINUS-CARPIO ; GENE-EXPRESSION
收录类别	SCI
语种	英语
WOS记录号	WOS:000381537000004
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/28353]
专题	水生生物研究所_水生生物多样性与资源保护研究中心_学位论文
作者单位	1.Sichuan Agr Univ, Inst Anim Nutr, Chengdu 611130, Sichuan, Peoples R China 2.Sichuan Agr Univ, Fish Nutr & Safety Prod Univ Key Lab Sichuan Prov, Chengdu 611130, Peoples R China 3.Sichuan Agr Univ, China Minist Educ, Key Lab Anim Dis Resistance Nutr, Chengdu 611130, Peoples R China 4.Sichuan Acad Anim Sci, Inst Anim Nutr, Chengdu 610066, Peoples R China 5.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Ni, Pei-Jun,Jiang, Wei-Dan,Wu, Pei,et al. Dietary low or excess levels of lipids reduced growth performance, and impaired immune function and structure of head kidney, spleen and skin in young grass carp (Ctenopharyngodon idella) under the infection of Aeromonas hydrophila[J]. FISH & SHELLFISH IMMUNOLOGY,2016,55:28-47.
APA	Ni, Pei-Jun.,Jiang, Wei-Dan.,Wu, Pei.,Liu, Yang.,Kuang, Sheng-Yao.,...&Feng, Lin.(2016).Dietary low or excess levels of lipids reduced growth performance, and impaired immune function and structure of head kidney, spleen and skin in young grass carp (Ctenopharyngodon idella) under the infection of Aeromonas hydrophila.FISH & SHELLFISH IMMUNOLOGY,55,28-47.
MLA	Ni, Pei-Jun,et al."Dietary low or excess levels of lipids reduced growth performance, and impaired immune function and structure of head kidney, spleen and skin in young grass carp (Ctenopharyngodon idella) under the infection of Aeromonas hydrophila".FISH & SHELLFISH IMMUNOLOGY 55(2016):28-47.