Ammonia exposure induces oxidative stress, endoplasmic reticulum stress and apoptosis in hepatopancreas of pacific white shrimp (Litopenaeus vannamei)

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Ammonia exposure induces oxidative stress, endoplasmic reticulum stress and apoptosis in hepatopancreas of pacific white shrimp (Litopenaeus vannamei)
	Liang, Zhongxiu 1; Liu, Rui 1; Zhao, Depeng 3; Wang, Lingling 2; Sun, Mingzhe1 ; Wang, Mengqiang1 ; Song, Linsheng 2
刊名	FISH & SHELLFISH IMMUNOLOGY
	2016-07-01
卷号	54 页码:523-528
关键词	Litopenaeus vannamei Ammonia exposure Oxidative stress Endoplasmic reticulum stress Apoptosis
英文摘要	Ammonia is one of major environmental pollutants in the aquatic system that poses a great threat to the survival of shrimp. In the present study, the mRNA expression of endoplasmic reticulum (ER) stress marker and unfolded protein response (UPR) related genes, as well as the change of redox enzyme and apoptosis were investigated in hepatopancreas of the pacific white shrimp, Litopenaeus vannamei after the exposure of 20 mg L-1 total ammonia nitrogen (TAN). Compared with the control group, the superoxide dismutase (SOD) activity in hepatopancreas decreased significantly (p < 0.05) at 96 h, whereas the malonyldialdehyde (MDA) concentration increased significantly (p < 0.05). The mRNA expression levels of ER stress marker-immunoglobulin heavy chain binding protein (Bip) gene and key UPR related genes including activating transcription factor 4 (ATF4) and the spliced form of X box binding protein 1 (XBP1) increased significantly (p < 0.05) in hepatopancreas at 96 h after exposure to ammonia. In addition, apoptosis was observed obviously in the hepatopancreas of L. vannamei after exposure to ammonia by the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay. The results indicated that ammonia exposure could induce oxidative stress, which further caused ER stress and apoptosis in hepatopancreas of L. vannamei. (C) 2016 Elsevier Ltd. All rights reserved.
收录类别	SCI
语种	英语
WOS记录号	WOS:000378451700061
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.qdio.ac.cn/handle/337002/130999]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Dalian Ocean Univ, Key Lab Mariculture Stock Enhancement North China, Minist Agr, Dalian 116023, Peoples R China 3.Dalian Polytech Univ, Dalian 116034, Peoples R China
推荐引用方式 GB/T 7714	Liang, Zhongxiu,Liu, Rui,Zhao, Depeng,et al. Ammonia exposure induces oxidative stress, endoplasmic reticulum stress and apoptosis in hepatopancreas of pacific white shrimp (Litopenaeus vannamei)[J]. FISH & SHELLFISH IMMUNOLOGY,2016,54:523-528.
APA	Liang, Zhongxiu.,Liu, Rui.,Zhao, Depeng.,Wang, Lingling.,Sun, Mingzhe.,...&Song, Linsheng.(2016).Ammonia exposure induces oxidative stress, endoplasmic reticulum stress and apoptosis in hepatopancreas of pacific white shrimp (Litopenaeus vannamei).FISH & SHELLFISH IMMUNOLOGY,54,523-528.
MLA	Liang, Zhongxiu,et al."Ammonia exposure induces oxidative stress, endoplasmic reticulum stress and apoptosis in hepatopancreas of pacific white shrimp (Litopenaeus vannamei)".FISH & SHELLFISH IMMUNOLOGY 54(2016):523-528.