Accumulation of -aminobutyric acid by Enterococcus avium 9184 in scallop solution in a two-stage fermentation strategy

	Accumulation of -aminobutyric acid by Enterococcus avium 9184 in scallop solution in a two-stage fermentation strategy
	Yang, Haoyue1,2 ; Xing, Ronge1 ; Hu, Linfeng 1; Liu, Song 1; Li, Pengcheng1
刊名	MICROBIAL BIOTECHNOLOGY
	2016-07-01
卷号	9 期号:4 页码:478-485
英文摘要	In this study, a new bacterial strain having a high ability to produce -aminobutyric acid (GABA) was isolated from naturally fermented scallop solution and was identified as Enterococcus avium. To the best of our knowledge, this is the first study to prove that E.avium possesses glutamate decarboxylase activity. The strain was then mutagenized with UV radiation and was designated as E.avium 9184. Scallop solution was used as the culture medium to produce GABA. A two-stage fermentation strategy was applied to accumulate GABA. In the first stage, cell growth was regulated. Optimum conditions for cell growth were pH, 6.5; temperature, 37 degrees C; and glucose concentration, 10gL(-1). This produced a maximum dry cell mass of 2.10gL(-1). In the second stage, GABA formation was regulated. GABA concentration reached 3.71gL(-1) at 96h pH 6.0, 37 degrees C and initial l-monosodium glutamate concentration of 10gL(-1). Thus, compared with traditional one-stage fermentation, the two-stage fermentation significantly increased GABA accumulation. These results provide preliminary data to produce GABA using E.avium and also provide a new approach to process and utilize shellfish.
收录类别	SCI
语种	英语
WOS记录号	WOS:000379689900006
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.qdio.ac.cn/handle/337002/130947]
专题	海洋研究所_海洋生物技术研发中心
作者单位	1.Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Yang, Haoyue,Xing, Ronge,Hu, Linfeng,et al. Accumulation of -aminobutyric acid by Enterococcus avium 9184 in scallop solution in a two-stage fermentation strategy[J]. MICROBIAL BIOTECHNOLOGY,2016,9(4):478-485.
APA	Yang, Haoyue,Xing, Ronge,Hu, Linfeng,Liu, Song,&Li, Pengcheng.(2016).Accumulation of -aminobutyric acid by Enterococcus avium 9184 in scallop solution in a two-stage fermentation strategy.MICROBIAL BIOTECHNOLOGY,9(4),478-485.
MLA	Yang, Haoyue,et al."Accumulation of -aminobutyric acid by Enterococcus avium 9184 in scallop solution in a two-stage fermentation strategy".MICROBIAL BIOTECHNOLOGY 9.4(2016):478-485.