Expression Characterization of Stress Genes Under High and Low Temperature Stresses in the Pacific Oyster, Crassostrea gigas

	Expression Characterization of Stress Genes Under High and Low Temperature Stresses in the Pacific Oyster, Crassostrea gigas
	Zhu, Qihui1,2,4 ; Zhang, Linlin 1,3,4; Li, Li 1,4,5; Que, Huayong 1,3,4; Zhang, Guofan 1,3,4
刊名	MARINE BIOTECHNOLOGY
	2016-04-01
卷号	18 期号:2 页码:176-188
关键词	Oysters Temperature stress Stress proteins Gene expression Adaptation
英文摘要	As a characteristic sessile inhabitant of the intertidal zone, the Pacific oyster Crassostrea gigas occupies one of the most physically stressful environments on earth. With high exposure to terrestrial conditions, oysters must tolerate broad fluctuations in temperature range. However, oysters' cellular and molecular responses to temperature stresses have not been fully characterized. Here, we analyzed oyster transcriptome data under high and low temperatures. We also identified over 30 key temperature stress-responsive candidate genes, which encoded stress proteins such as heat shock proteins and apoptosis-associated proteins. The expression characterization of these genes under short-term cold and hot environments (5 and 35 A degrees C) and long-term cold environments (5 A degrees C) was detected by quantitative real-time PCR. Most of these genes reached expression peaks during the recovery stage after 24 h of heat stress, and these genes were greatly induced around day 3 in long-term cold stress while responded little to short-term cold stress. In addition, in the second heat stress after 2 days of recovery, oysters showed milder expression in these genes and a lower mortality rate, which indicated the existence of plasticity in the oyster's response to heat stress. We confirmed that homeostatic flexibility and anti-apoptosis might be crucial centers of temperature stress responses in oysters. Furthermore, we analyzed stress gene families in 11 different species and found that the linage-specific expansion of stress genes might be implicated in adaptive evolution. These results indicated that both plasticity and evolution played an important role in the stress response adaptation of oysters.
收录类别	SCI
语种	英语
WOS记录号	WOS:000371809800003
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/126193]
专题	海洋研究所_海洋生物技术研发中心
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao, Peoples R China 5.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries & Aquaculture, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Zhu, Qihui,Zhang, Linlin,Li, Li,et al. Expression Characterization of Stress Genes Under High and Low Temperature Stresses in the Pacific Oyster, Crassostrea gigas[J]. MARINE BIOTECHNOLOGY,2016,18(2):176-188.
APA	Zhu, Qihui,Zhang, Linlin,Li, Li,Que, Huayong,&Zhang, Guofan.(2016).Expression Characterization of Stress Genes Under High and Low Temperature Stresses in the Pacific Oyster, Crassostrea gigas.MARINE BIOTECHNOLOGY,18(2),176-188.
MLA	Zhu, Qihui,et al."Expression Characterization of Stress Genes Under High and Low Temperature Stresses in the Pacific Oyster, Crassostrea gigas".MARINE BIOTECHNOLOGY 18.2(2016):176-188.