Invertase-mediated system for simple and rapid detection of pathogen

CORC > 海洋研究所 > 中国科学院海洋研究所 > 海洋腐蚀与防护研究发展中心

	Invertase-mediated system for simple and rapid detection of pathogen
	Wan, Yi; Qi, Peng; Zeng, Yan; Sun, Yan; Zhang, Dun
刊名	SENSORS AND ACTUATORS B-CHEMICAL
	2016-10-05
卷号	233 页码:454-458
关键词	Commercial glucose meter Bacteria detection Invertase Aptamer
英文摘要	Portable, low-cost and quantitative analysis of pathogen at home and in the field remain a key challenge in medical diagnostics and food safety. Despite many years of research, very few such tools are available. Motivated by present clinical requirements, pocket-sized device, such as commercial glucose meter (CGM), have emerged. Taking advantage of the CGM, we develop an approach to quantify bacteria. Molecular recognition elements, such as aptamer, coupled with a signal amplification method based on invertase-promoted hydrolysis of sucrose for the sensitive and selective detection of bacteria. This paper aims to develop a portable device combined with enzyme-mediated enhancement for biological/chemical analyte detection. A linear relationship between the response and the logarithm of the Staphylococcus aureus concentration using this method was obtained for concentrations ranging from 1.2 x 10(5) to 1.2 x 10(8) cfu mL(-1), with a detection limit of 1.0 x 10(5) cfu mL(-1). This study demonstrates an approach for bacteria detection and also provides a powerful platform to monitor bacterial infection, thus rendering potential broad antibacterial applications and bacteria diagnosis. (C) 2016 Elsevier B.V. All rights reserved.
收录类别	SCI
语种	英语
WOS记录号	WOS:000376384100034
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.qdio.ac.cn/handle/337002/131084]
专题	海洋研究所_海洋腐蚀与防护研究发展中心
作者单位	Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, 7 Nanhai Rd, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Wan, Yi,Qi, Peng,Zeng, Yan,et al. Invertase-mediated system for simple and rapid detection of pathogen[J]. SENSORS AND ACTUATORS B-CHEMICAL,2016,233:454-458.
APA	Wan, Yi,Qi, Peng,Zeng, Yan,Sun, Yan,&Zhang, Dun.(2016).Invertase-mediated system for simple and rapid detection of pathogen.SENSORS AND ACTUATORS B-CHEMICAL,233,454-458.
MLA	Wan, Yi,et al."Invertase-mediated system for simple and rapid detection of pathogen".SENSORS AND ACTUATORS B-CHEMICAL 233(2016):454-458.