Pulsed galvanostatic control of a solid-contact ion-selective electrode for potentiometric biosensing of microcystin-LR

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室

	Pulsed galvanostatic control of a solid-contact ion-selective electrode for potentiometric biosensing of microcystin-LR
	Yu, NN ; Ding, JW; Wang, WW ; Wang, XD ; Qin, W
刊名	SENSORS AND ACTUATORS B-CHEMICAL
	2016-07-01
卷号	230 页码:785-790
关键词	Chronopotentiometry Solid-contact ion-selective electrodes Protein phosphatases Microcystin-LR
ISSN号	0925-4005
通讯作者	Ding, JW (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Shandong Prov Key Lab Coastal Environm Proc, Key Lab Coastal Environm Proc & Ecol Remediat,YIC, Yantai 264003, Shandong, Peoples R China. ; Wang, XD (reprint author), Wenzhou Med Univ, Dept Environm Sci, Key Lab Watershed Sci & Hlth Zhejiang Prov, Wenzhou 325035, Peoples R China. jwding@yic.ac.cn ; zjuwxd@163.com
产权排序	[Yu, Nana; Ding, Jiawang; Qin, Wei] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Shandong Prov Key Lab Coastal Environm Proc, Key Lab Coastal Environm Proc & Ecol Remediat,YIC, Yantai 264003, Shandong, Peoples R China; [Yu, Nana; Wang, Wenwei; Wang, Xuedong] Wenzhou Med Univ, Dept Environm Sci, Key Lab Watershed Sci & Hlth Zhejiang Prov, Wenzhou 325035, Peoples R China
英文摘要	We report here on the development of a chronopotentiometric assay for microcystin-LR based on enzymatic inhibition. The inhibition of protein phosphatase by microcystin-LR can be sensed potentiometrically by using 4-nitrophenyl phosphate as an enzyme substrate. A solid-contact ion-selective electrode (ISE) with poly(3,4-ethylenedioxythiophene)-poly(styrenesulfonate) as a transduction layer has been designed for potentiometric biosensing using the pulsed galvanastatic technique. By applying an anodic current, the enzymatic generated p-nitrophenol can be extracted into the polymeric membrane with tetradodecylammonium tetrakis(4-chlorophenyl)-borate to produce the chronopotentiometric signal. Meanwhile, a controlled voltage was applied to refresh the membrane for multiple consecutive measurements. The proposed potentiometric assay showed a linear response for microcystin-LR in the range 1-100 mu g/L with a detection limit of 0.5 mu g/L (3 sigma). We believe that the proposed method can be employed for sensitive, rapid and reliable determination of analytes involved in enzyme inhibition. (C) 2016 Elsevier B.V. All rights reserved.
研究领域[WOS]	Chemistry ; Electrochemistry ; Instruments & Instrumentation
关键词[WOS]	LABEL-FREE ; MEMBRANE ELECTRODES ; SENSITIVE DETECTION ; INHIBITION ASSAY ; ELECTROCHEMICAL DETECTION ; PROTEIN PHOSPHATASES ; ENZYME ; WATER ; QUANTIFICATION ; IMMUNOSENSOR
收录类别	SCI
语种	英语
WOS记录号	WOS:000374329300100
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/17056]
专题	烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Shandong Prov Key Lab Coastal Environm Proc, Key Lab Coastal Environm Proc & Ecol Remediat,YIC 2.Wenzhou Med Univ, Dept Environm Sci, Key Lab Watershed Sci & Hlth Zhejiang Prov
推荐引用方式 GB/T 7714	Yu, NN,Ding, JW,Wang, WW,et al. Pulsed galvanostatic control of a solid-contact ion-selective electrode for potentiometric biosensing of microcystin-LR[J]. SENSORS AND ACTUATORS B-CHEMICAL,2016,230:785-790.
APA	Yu, NN,Ding, JW,Wang, WW,Wang, XD,&Qin, W.(2016).Pulsed galvanostatic control of a solid-contact ion-selective electrode for potentiometric biosensing of microcystin-LR.SENSORS AND ACTUATORS B-CHEMICAL,230,785-790.
MLA	Yu, NN,et al."Pulsed galvanostatic control of a solid-contact ion-selective electrode for potentiometric biosensing of microcystin-LR".SENSORS AND ACTUATORS B-CHEMICAL 230(2016):785-790.