Enhanced green fluorescent protein (egfp) gene expression in Tetraselmis subcordiformis chloroplast with endogenous regulators

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	Enhanced green fluorescent protein (egfp) gene expression in Tetraselmis subcordiformis chloroplast with endogenous regulators
	Cui, YL; Zhao, JL ; Hou, SC ; Qin, S
刊名	WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
	2016-05-01
卷号	32 期号:5
关键词	Tetraselmis subcordiformis Chloroplast Promoters Bombardment
ISSN号	0959-3993
通讯作者	Qin, S (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resource Utilizat, Chunhui Rd 17, Yantai 264003, Shandong, Peoples R China. yulincui@yic.ac.cn ; sqin@yic.ac.cn
产权排序	[Cui, Yulin; Zhao, Jialin; Qin, Song] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resource Utilizat, Chunhui Rd 17, Yantai 264003, Shandong, Peoples R China; [Zhao, Jialin] Univ Chinese Acad Sci, Yuquan Rd 19, Beijing 100049, Peoples R China; [Hou, Shichang] Yantai Univ, Coll Life Sci, Qingquan Rd 30, Yantai 264005, Shandong, Peoples R China
英文摘要	On the basis of fundamental genetic transformation technologies, the goal of this study was to optimize Tetraselmis subcordiformis chloroplast transformation through the use of endogenous regulators. The genes rrn16S, rbcL, psbA, and psbC are commonly highly expressed in chloroplasts, and the regulators of these genes are often used in chloroplast transformation. For lack of a known chloroplast genome sequence, the genome-walking method was used here to obtain full sequences of T. subcordiformis endogenous regulators. The resulting regulators, including three promoters, two terminators, and a ribosome combination sequence, were inserted into the previously constructed plasmid pPSC-R, with the egfp gene included as a reporter gene, and five chloroplast expression vectors prepared. These vectors were successfully transformed into T. subcordiformis by particle bombardment and the efficiency of each vector tested by assessing EGFP fluorescence via microscopy. The results showed that these vectors exhibited higher efficiency than the former vector pPSC-G carrying exogenous regulators, and the vector pRFA with Prrn, psbA-5'RE, and TpsbA showed the highest efficiency. This research provides a set of effective endogenous regulators for T. subcordiformis and will facilitate future fundamental studies of this alga.
研究领域[WOS]	Biotechnology & Applied Microbiology
关键词[WOS]	ALGA PLATYMONAS-SUBCORDIFORMIS ; PLASTID TRANSFORMATION ; VECTOR ; CONSTRUCTION ; SEQUENCES ; ELEMENTS ; TOBACCO ; OPERON
收录类别	SCI
语种	英语
WOS记录号	WOS:000375089400012
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/17058]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resource Utilizat 2.Univ Chinese Acad Sci 3.Yantai Univ, Coll Life Sci
推荐引用方式 GB/T 7714	Cui, YL,Zhao, JL,Hou, SC,et al. Enhanced green fluorescent protein (egfp) gene expression in Tetraselmis subcordiformis chloroplast with endogenous regulators[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,2016,32(5).
APA	Cui, YL,Zhao, JL,Hou, SC,&Qin, S.(2016).Enhanced green fluorescent protein (egfp) gene expression in Tetraselmis subcordiformis chloroplast with endogenous regulators.WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,32(5).
MLA	Cui, YL,et al."Enhanced green fluorescent protein (egfp) gene expression in Tetraselmis subcordiformis chloroplast with endogenous regulators".WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 32.5(2016).