Visualization of the 3D structures of small organisms via LED-SIM
Ruan, Yongying1,3; Dan, Dan2; Zhang, Mengna1,3; Bai, Ming1; Lei, Ming2; Yao, Baoli2; Yang, Xingke1
刊名frontiers in zoology
2016-06-14
卷号13
关键词3D imaging Micro-structures Structured illumination microscopy LED illumination Microscopy Morphology
ISSN号1742-9994
产权排序2
英文摘要background: innovative new techniques that aid in the visualization of microscopic anatomical structures have improved our understanding of organismal biology significantly. it is often challenging to observe internal 3d structures, despite the use of techniques such as confocal laser scanning microscopy (clsm), micro-computed tomography (micro-ct), magnetic resonance imaging (mri), focused ion beam scanning electron microscopy (fib-sem) and others. in the current paper, we assess led-sim (dmd-based led-illumination structured illumination microscopy), which facilitates the acquisition of nano-and micro-3d structures of small organisms in a high-resolution format (500 nm in the xy-plane and 930 nm along the z-axis).
WOS标题词science & technology ; life sciences & biomedicine
类目[WOS]zoology
研究领域[WOS]zoology
关键词[WOS]plane illumination microscopy ; fluorescence microscopy ; scanning microscope ; resolution ; tomography ; limit ; excitation ; cells
收录类别SCI
语种英语
WOS记录号WOS:000379681100001
内容类型期刊论文
源URL[http://ir.opt.ac.cn/handle/181661/28180]  
专题西安光学精密机械研究所_瞬态光学技术国家重点实验室
作者单位1.Chinese Acad Sci, Inst Zool, Key Lab Zool Systemat & Evolut, Beijing 100101, Peoples R China
2.Chinese Acad Sci, Xian Inst Opt & Precis Mech, State Key Lab Transient Opt & Photon, Xian 710119, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
推荐引用方式
GB/T 7714
Ruan, Yongying,Dan, Dan,Zhang, Mengna,et al. Visualization of the 3D structures of small organisms via LED-SIM[J]. frontiers in zoology,2016,13.
APA Ruan, Yongying.,Dan, Dan.,Zhang, Mengna.,Bai, Ming.,Lei, Ming.,...&Yang, Xingke.(2016).Visualization of the 3D structures of small organisms via LED-SIM.frontiers in zoology,13.
MLA Ruan, Yongying,et al."Visualization of the 3D structures of small organisms via LED-SIM".frontiers in zoology 13(2016).
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