Biothiol Xenon MRI Sensor Based on Thiol-Addition Reaction

CORC > 武汉物理与数学研究所 > 中国科学院武汉物理与数学研究所 > 磁共振基础研究部

	Biothiol Xenon MRI Sensor Based on Thiol-Addition Reaction
	Yang, Shengjun 1; Jiang, Weiping 1; Ren, Lili 1; Yuan, Yaping 1; Zhang, Bin 1; Luo, Qing 1; Guo, Qianni 1; Bouchard, Louis-S.2; Liu, Maili 1; Zhou, Xin 1
刊名	ANALYTICAL CHEMISTRY
	2016-06-07
卷号	88 期号:11 页码:5835-5840
英文摘要	Biothiols such as cysteine (Cys), homocysteine (Hcy), and glutathione (GSH) play an important role in regulating the vital functions of living organisms. Knowledge of their biodistribution in real-time could help diagnose a variety of conditions. However, existing methods of biothiol detection are invasive and require assays. Herein we report a molecular biosensor for biothiol detection using the nuclear spin resonance of Xe-129. The Xe-129 biosensor consists of a cryptophane cage encapsulating a xenon atom and an acrylate group. The latter serves as a reactive site to covalently bond biothiols through a thiol-addition reaction. The biosensor enables discrimination of Cys from Hcy and GSH through the chemical shift and average reaction rate. This biosensor can be detected at a concentration of 10 mu M in a single scan and it has been applied to detect biothiols in bovine serum solution. Our results indicate that this biosensor is a promising tool for the real-time imaging of biothiol distributions.
WOS标题词	Science & Technology ; Physical Sciences
类目[WOS]	Chemistry, Analytical
研究领域[WOS]	Chemistry
关键词[WOS]	NUCLEAR-MAGNETIC-RESONANCE ; HUMAN CARBONIC-ANHYDRASE ; XE-129 NMR ; SELECTIVE DETECTION ; MASS-SPECTROMETRY ; LIVING CELLS ; IN-VIVO ; FLUORESCENCE DETECTION ; FUNCTIONALIZED XENON ; CYSTEINE
收录类别	SCI
语种	英语
WOS记录号	WOS:000377631000041
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/9406]
专题	武汉物理与数学研究所_磁共振基础研究部
作者单位	1.Chinese Acad Sci, State Key Lab Magnet Resonance & Atom & Mol Phys, Key Lab Magnet Resonance Biol Syst, Natl Ctr Magnet Resonance Wuhan,Wuhan Inst Phys &, Wuhan 430071, Peoples R China 2.Univ Calif Los Angeles, Dept Chem & Biochem, Calif NanoSyst Inst, Inst Mol Biol, Los Angeles, CA 90095 USA
推荐引用方式 GB/T 7714	Yang, Shengjun,Jiang, Weiping,Ren, Lili,et al. Biothiol Xenon MRI Sensor Based on Thiol-Addition Reaction[J]. ANALYTICAL CHEMISTRY,2016,88(11):5835-5840.
APA	Yang, Shengjun.,Jiang, Weiping.,Ren, Lili.,Yuan, Yaping.,Zhang, Bin.,...&Zhou, Xin.(2016).Biothiol Xenon MRI Sensor Based on Thiol-Addition Reaction.ANALYTICAL CHEMISTRY,88(11),5835-5840.
MLA	Yang, Shengjun,et al."Biothiol Xenon MRI Sensor Based on Thiol-Addition Reaction".ANALYTICAL CHEMISTRY 88.11(2016):5835-5840.