TBK1-like transcript negatively regulates the production of IFN and IFN-stimulated genes through RLRs-MAVS-TBK1 pathway

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	TBK1-like transcript negatively regulates the production of IFN and IFN-stimulated genes through RLRs-MAVS-TBK1 pathway
	Zhang, Lin 1,2; Chen, Wen Qin 3; Hu, Yi Wei 2,4; Wu, Xiao Man 2,4; Nie, P.2; Chang, Ming Xian 2
刊名	FISH & SHELLFISH IMMUNOLOGY
	2016-07-01
卷号	54 页码:135-143
关键词	Zebrafish TBK1 TBK1-like transcript RIG-I-Like receptors IFN Innate immune signaling
ISSN号	1050-4648
英文摘要	TANK-binding kinase 1 (TBK1) is an essential serine/threonine-protein kinase required for Toll-like receptor (TLR)- and retinoic acid-inducible gene I (RIG-I)-mediated induction of type I IFN and host antiviral defense. In the present study, TBK1-like transcript, namely TBK1L, was cloned from zebrafish. Compared with TBK1, TBK1L contains an incomplete S_TKc domain, and lacks UBL_TBK1iike domain. Realtime PCR showed that TBK1L was constitutively produced in embryos, early larvae and ZF4 cells, and unchanged in ZF4 cells following SVCV infection. Overexpression of TBK1 but not TBK1L resulted in significant activation of zebrafish IFNI and IFN3 promoters. Similarly, TBK1L had little impact on the antiviral state of the cells. However, the overexpression of TBK1L negatively regulated the induction of zebrafish IFNI and/or IFN3 promoters mediated by the retinoic acid-inducible gene I-like receptors (RLRs), MAVS and TBK1. In addition, the overexpression of TBK1L in zebrafish embryos led to the decreased production of many IFN-stimulated genes induced by TBK1. Collectively, these data support that zebrafish TBK1L negatively regulates RLRs-MAVS-TBK1 pathway. (C) 2016 Elsevier Ltd. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
研究领域[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
关键词[WOS]	INNATE IMMUNE-RESPONSE ; I-LIKE RECEPTORS ; BINDING KINASE 1 ; ANTIVIRAL RESPONSE ; RIG-I ; AUTOIMMUNE-DISEASES ; SIGNALING PATHWAY ; TBK1 ; INTERFERON ; ZEBRAFISH
收录类别	SCI
语种	英语
WOS记录号	WOS:000378451700016
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/28011]
专题	水生生物研究所_藻类生物学及应用研究中心
作者单位	1.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Hubei Province, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China 3.Hubei Vocat Coll Biotechnol, Wuhan 430070, Hubei Province, Peoples R China 4.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Lin,Chen, Wen Qin,Hu, Yi Wei,et al. TBK1-like transcript negatively regulates the production of IFN and IFN-stimulated genes through RLRs-MAVS-TBK1 pathway[J]. FISH & SHELLFISH IMMUNOLOGY,2016,54:135-143.
APA	Zhang, Lin,Chen, Wen Qin,Hu, Yi Wei,Wu, Xiao Man,Nie, P.,&Chang, Ming Xian.(2016).TBK1-like transcript negatively regulates the production of IFN and IFN-stimulated genes through RLRs-MAVS-TBK1 pathway.FISH & SHELLFISH IMMUNOLOGY,54,135-143.
MLA	Zhang, Lin,et al."TBK1-like transcript negatively regulates the production of IFN and IFN-stimulated genes through RLRs-MAVS-TBK1 pathway".FISH & SHELLFISH IMMUNOLOGY 54(2016):135-143.