Transcriptome analysis of phycocyanin inhibitory effects on SKOV-3 cell proliferation

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	Transcriptome analysis of phycocyanin inhibitory effects on SKOV-3 cell proliferation
	Ying, Jun 1; Wang, Jian 1,2; Ji, Huijuan 1,2; Lin, Chaoqing 1,2; Pan, Ruowang 3; Zhou, Li 1; Song, Yulong 4,5; Zhang, Enyong 3; Ren, Ping 1; Chen, Jishun 1
刊名	GENE
	2016-07-01
卷号	585 期号:1 页码:58-64
关键词	Phycocyanin SKOV-3 cell Cell proliferation RNA-seq qRT-PCR
ISSN号	0378-1119
英文摘要	Phycocyanin (PC) from Spirulina platensis has inhibitory effects on tumor cell growth. In this research, the transcriptome study was designed to investigate the underlying molecular mechanisms of PC inhibition on human ovarian cancer cell SKOV-3 proliferation. The PC IC50 was 216.6 mu M and 163.8 mu M for 24 h and 48 h exposure, respectively, as determined by CCK-8 assay. The morphological changes of SKOV-3 cells after PC exposure were recorded using HE staining. Cells arrested in G(2)/M stages as determined by flow cytometry. The transcriptome analysis showed that 2031 genes (with > three-fold differences) were differentially expressed between the untreated and the PC-treated cells, including 1065 up-regulated and 966 down-regulated genes. Gene ontology and KEGG pathway analysis identified 18 classical pathways that were remarkably enriched, such as neurotrophin signaling pathway, VEGF signaling pathway and P53 signaling pathway. ciPCR results further showed that PTPN12, S100A2, RPL26, and LAMA3 increased while HNRNPA1P10 decreased in PC-treated cells. Molecules and genes in those pathways may be potential targets to develop treatments for ovarian cancer. (C) 2016 Elsevier B.V. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Genetics & Heredity
研究领域[WOS]	Genetics & Heredity
关键词[WOS]	C-PHYCOCYANIN ; GENE-EXPRESSION ; IN-VITRO ; CANCER ; APOPTOSIS ; THERAPY ; VIVO
收录类别	SCI
语种	英语
WOS记录号	WOS:000375519400009
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/27965]
专题	水生生物研究所_水生生物多样性与资源保护研究中心
作者单位	1.Wenzhou Med Univ, Sch Lab Med & Life Sci, Inst Biomed Informat, Wenzhou 325035, Peoples R China 2.Wenzhou Med Univ, Affiliated Hosp 2, Wenzhou 325000, Peoples R China 3.PLA, Hosp 118, Wenzhou 325000, Peoples R China 4.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China 5.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Ying, Jun,Wang, Jian,Ji, Huijuan,et al. Transcriptome analysis of phycocyanin inhibitory effects on SKOV-3 cell proliferation[J]. GENE,2016,585(1):58-64.
APA	Ying, Jun.,Wang, Jian.,Ji, Huijuan.,Lin, Chaoqing.,Pan, Ruowang.,...&Li, Peizhen.(2016).Transcriptome analysis of phycocyanin inhibitory effects on SKOV-3 cell proliferation.GENE,585(1),58-64.
MLA	Ying, Jun,et al."Transcriptome analysis of phycocyanin inhibitory effects on SKOV-3 cell proliferation".GENE 585.1(2016):58-64.