| 题名 | 肝细胞功能活性在线定性检测系统的研究; 肝细胞功能活性在线定性检测系统的研究 |
| 作者 | 1项红升,电工研究所 |
| 学位类别 | 硕士 |
| 答辩日期 | 2005 |
| 授予单位 | 中国科学院电工研究所 |
| 导师 | 1李明,电工研究所 |
| 关键词 | 生物人工肝 肝细胞功能检测 尿素 白蛋白 发光二极管 BALS LED online qualitative fast detecting Monte Carlo |
| 其他题名 | 肝细胞功能活性在线定性检测系统的研究 |
| 学位专业 | 电工理论与新技术 |
| 中文摘要 | 本文主要对应用于生物人工肝体外支持系统（BioartificialLiverSupportSystem，BAL）的肝细胞功能活性在线定性快速检测进行了系统的初步研究。在此基础上设计实现了该检测系统的主要功能。对BAL系统中的肝细胞进行功能活性的在线定性检测是保证BAL正确行使功能的重要前提。本文对现有的肝细胞功能活性检测技术，从原理和方法上进行了系统的分析比较；并从中筛选出适宜进行在线快速定性检测的生化分析指标和相应的检测方法。本文依据白蛋白和尿素指标检测特点，设计了以光学比色原理为基础的在线定性检测分析系统。在初步系统中，实现了光电基座的设计，完成了以MEGA16单片机为主控单元、以OPT301集成光电传感器为光电接收单元、以104BH智能液晶触摸屏终端为人机交互界面的光机电集成系统。以串行通讯方式实现与显示终端和上位机的通讯。本课题的主要特点包括：在人工肝生化检测中，首次应用超亮发光二极管作为测量光源，由此设计完成简约小巧的光机结构，该光机结构能够方便的集成在BALS系统中，实现在线定性检测的功能。采用MEGA16主控制器，集成了10位模数转换、UART、JTAG等，以极低的硬件成本实现了集中的检测控制。本课题对光电器件和光电转换结构进行了蒙特卡罗仿真分析。通过仿真确定了优化的位置和光学属性数据。另外，本文对所设计的检测装置与日立公司的UV-280D紫外可见分光光度计进行了对比试验，试验证明，两者定性测量精度一致，本课题装置实现了其难以实现的在线快速定性检测功能，而且可拆装的光机结构满足诸多现场小空间的测量应用要求。 Bio-artificial Liver Support System (BALSS) is essential to the survival of suffers with heavy liver diseases such as acute liver failure. Hepatocytes' viability and liver-specific differentiational functions should be considered to the BALSS. This dissertation focused on the research of detecting hepatocytes' viability and functioninbio-reactor of BALSS with a novel system.Firstly, the principles and methods of cells' viability detecting were described. And two key bio-chemical indexes--urea and albumin were set as parameters. Tow kinds of ultra bright lighting emitting diodes (LED) used as lamp of colorimetry.Secondly, a novel monitoring system architecture based on LED and integrated optical-electric sensor was presented, which used red LED and yellow-green LED as measuring lamp to detect albumin and urea. The system includes three parts: sampling sub-system based on precision peristaltic pumps, photoelectric sub-system which includes two LEDS and cuvette and two OPT301 (optical-electric sensor), controlling units.Thirdly, LED module simulation based on Monte Carlo algorithm was demonstrated in detail. Some optimized parameters was given such as LED's encapsulation cannula out of LED > distance of several components after simulation applied by TRACEPRO and AS AP(professional software).Finally, a contrastive experiment was designed to test the system's performance. The result of experiment compared with UV-2800 spectrophotometer proved that this system satisfied the requirement of BALS, which can qualitative fast detecting online, sample culture during the running process of BALSS, qualitatively analyze Albumin and Urea quickly, and then display the two indexes, lets operators take measures .The most advanced point of this system is online qualitative detecting, without influencing system processing, without polluting to the system. Getting data quickly on-site, ease to adapt the system (such as changing cells, regulating environmental indexes). |
| 语种 | 中文 |
| 公开日期 | 2010-10-18 |
| 页码 | 72 |
| 内容类型 | 学位论文 |
| 源URL | [http://ir.iee.ac.cn/handle/311042/7375]  |
| 专题 | 电工研究所_其他部门_其他部门_硕士学位论文 |
| 推荐引用方式 GB/T 7714 | 1项红升,电工研究所. 肝细胞功能活性在线定性检测系统的研究, 肝细胞功能活性在线定性检测系统的研究[D]. 中国科学院电工研究所. 2005. |
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