Distribution of metalloproteins in hepatocellular carcinoma and surrounding tissues

	Distribution of metalloproteins in hepatocellular carcinoma and surrounding tissues
	Liu, YB; Li LN(李丽娜); Gao YX(高愈希); Chen CY(陈春英); Li B(李柏); He W(何伟); Huang YY(黄宇营); Chai ZF(柴之芳); Li, LN; Gao, YX
刊名	HEPATO-GASTROENTEROLOGY
	2007
卷号	54 期号:80 页码:2291-2296
关键词	hepatocellular carcinoma metalloproteins microsome cytosol synchrotron radiation X-ray fluorescence isoelectric focusing
通讯作者	[Li, Lina ; Gao, Yuxi ; Chen, Chunying ; Li, Bai ; He, Wei ; Huang, Yuying ; Chai, Zhifang] Chinese Acad Sci, Multidisciplinary Res Ctr, Inst High Energy Phys, Lab Nucl Analyt Tech, Beijing 100049, Peoples R China ; [Li, Lina ; Gao, Yuxi ; Chen, Chunying ; Li, Bai ; He, Wei ; Huang, Yuying ; Chai, Zhifang] Chinese Acad Sci, Multidisciplinary Res Ctr, Inst High Energy Phys, Lab Biol Effects Nanomat & Nanosafety, Beijing 100049, Peoples R China ; [Liu, Yingbin] Zhejiang Univ, Affiliated Hosp 2, Dept Surg, Hangzhou 310027, Peoples R China ; [Li, Lina] Baotou Med Coll, Dept Basic Med, Baotou, Peoples R China
英文摘要	Background/Aims: The aim of this study was to investigate the relationship between metalloproteins distributions and cancer development in human liver tissues. Methodology: Proteins in the cytosol and microsome of tumor and surrounding nontumor tissues from five individuals with HCC were separated with isoelectric focusing. Zinc, iron, copper, and manganese contents in protein bands were measured with synchrotron radiation X-ray fluorescence. Results: In the cytosol, the metal contents in protein bands from tumor tissues were generally less than those from corresponding nontumor tissues, especially in the Fe-containing bands with isoelectric points of 6.5 and 8.0, and Cu, Zn-containing band of 5.5. However, reversed metal contents in Fe, Cu-containing band of 4.0 and Zn-containing bands of 3.9 and 4.3 were observed. In microsomes, the metal contents of protein bands were generally similar between tumor and nontumor tissues, but the Fe content in tumor tissues was 2.5-fold lower in the band of 5.9, the Cu and Zn contents were 2 and 3.5-fold higher in the band of 9.3, respectively, compared to that of nontumor tissues. Conclusions: Metal distributions among the detected metal-containing bands are distinguishable between tumor and nontumor tissues. The difference of the distribution may be involved in the carcinogenesis of the liver tissues.
学科主题	Gastroenterology & Hepatology; Surgery
类目[WOS]	Gastroenterology & Hepatology ; Surgery
研究领域[WOS]	Gastroenterology & Hepatology ; Surgery
原文出处	SCI
语种	英语
WOS记录号	WOS:000251892800024
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/239711]
专题	高能物理研究所_院士高能物理研究所_实验物理中心
作者单位	中国科学院高能物理研究所
推荐引用方式 GB/T 7714	Liu, YB,Li LN,Gao YX,et al. Distribution of metalloproteins in hepatocellular carcinoma and surrounding tissues[J]. HEPATO-GASTROENTEROLOGY,2007,54(80):2291-2296.
APA	Liu, YB.,李丽娜.,高愈希.,陈春英.,李柏.,...&Chai, ZF.(2007).Distribution of metalloproteins in hepatocellular carcinoma and surrounding tissues.HEPATO-GASTROENTEROLOGY,54(80),2291-2296.
MLA	Liu, YB,et al."Distribution of metalloproteins in hepatocellular carcinoma and surrounding tissues".HEPATO-GASTROENTEROLOGY 54.80(2007):2291-2296.