Fullerene Nanoparticles Selectively Enter Oxidation-Damaged Cerebral Microvessel Endothelial Cells and Inhibit JNK-Related Apoptosis

	Fullerene Nanoparticles Selectively Enter Oxidation-Damaged Cerebral Microvessel Endothelial Cells and Inhibit JNK-Related Apoptosis
	Lao, F; Chen, L; Li, W; Ge, CC; Qu, Y; Sun, QM; Zhao, YL; Han, D; Chen, CY; Zhao YL(赵宇亮)
刊名	ACS NANO
	2009
卷号	3 期号:11 页码:3358-3368
关键词	C(60)(C(COOH)(2))(2) microvessel endothelial cells oxidative injury apoptosis JNK pathway
通讯作者	[Lao, Fang ; Chen, Long ; Qu, Ying ; Sun, Quanmei ; Han, Dong ; Chen, Chunying] Natl Ctr Nanosci & Technol NCNST, Beijing 100190, Peoples R China ; [Lao, Fang ; Chen, Long ; Li, Wei ; Ge, Cuicui ; Qu, Ying ; Sun, Quanmei ; Zhao, Yuliang ; Han, Dong ; Chen, Chunying] Chinese Acad Sci, NCNST, IHEP, Key Lab Biol Effects Nanomat & Nanosafety, Beijing 100049, Peoples R China
英文摘要	There is a dearth in fundamental cellular-level understanding of how nanoparticles interact with the cells of the blood brain barrier (BBB), particularly under the oxidative environment. The apoptosis of cerebral microvessel enclothelial cells (CMECs) induced by oxidative stress injury plays a key role in the dysfunction of BBB. By use of CMECs as an in vitro BBB model, we show for the first time that C(60)(C(COOH)(2))(2) nanoparticles can selectively enter oxidized CMECs rather than normal cells, and maintain CMECs integrity by attenuating H(2)O(2)-induced F-actin depolymerization via the observation of several state-of-the art microscopic techniques. Additionally, we have found that C(60)(C(COOH)(2))(2) nanoparticles greatly inhibit the apoptosis of CMECs induced by H(2)O(2), which is related to their modulation of the JNK pathway. C(60)(C(COOH)(2))(2) nanoparticles can regulate several downstream signaling events related to the JNK pathway, including reduction of JNK phosphorylation, activation of activator protein 1 (AP-1) and caspase-3, and inhibition of polyADP-ribose polymerase (PARP) cleavage and mitochondrial cytochrome c release. Our results indicate that C(60)(C(COOH)(2))(2) nanoparticles possess a novel ability of selectively entering oxidation-damaged cerebral enclothelial cells rather than normal enclothelial cells and then protecting them from apoptosis.
学科主题	Chemistry; Science & Technology - Other Topics; Materials Science
类目[WOS]	Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary
研究领域[WOS]	Chemistry ; Science & Technology - Other Topics ; Materials Science
原文出处	SCI
语种	英语
WOS记录号	WOS:000271951200006
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/239897]
专题	高能物理研究所_多学科研究中心高能物理研究所_理论物理室
作者单位	中国科学院高能物理研究所
推荐引用方式 GB/T 7714	Lao, F,Chen, L,Li, W,et al. Fullerene Nanoparticles Selectively Enter Oxidation-Damaged Cerebral Microvessel Endothelial Cells and Inhibit JNK-Related Apoptosis[J]. ACS NANO,2009,3(11):3358-3368.
APA	Lao, F.,Chen, L.,Li, W.,Ge, CC.,Qu, Y.,...&赵宇亮.(2009).Fullerene Nanoparticles Selectively Enter Oxidation-Damaged Cerebral Microvessel Endothelial Cells and Inhibit JNK-Related Apoptosis.ACS NANO,3(11),3358-3368.
MLA	Lao, F,et al."Fullerene Nanoparticles Selectively Enter Oxidation-Damaged Cerebral Microvessel Endothelial Cells and Inhibit JNK-Related Apoptosis".ACS NANO 3.11(2009):3358-3368.