Recursive direct phasing with reference-beam diffraction

	Recursive direct phasing with reference-beam diffraction
	Shen, Q; Wang J(王俊); Wang, J
刊名	ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
	2003
卷号	59 期号:5 页码:809-814
通讯作者	Cornell Univ, Cornell High Energy Synchrotron Source, Ithaca, NY 14853 USA ; Cornell Univ, Dept Mat Sci & Engn, Ithaca, NY 14853 USA ; Beijing Synchrotron Radiat Facil, Inst High Energy Phys, Beijing, Peoples R China
英文摘要	The reference-beam diffraction technique provides a practical way to measure a large number of triplet phases in a standard oscillating-crystal diffraction experiment for protein crystals. The triplet-phase data set from such reference-beam measurements contains a unique phase-ocurrence pattern that leads to a new recursive phasing algorithm for the individual structure-factor phases. Application of the new algorithm is demonstrated for tetragonal lysozyme using 7360 triplet phases measured in a reference-beam experiment with a median phase discrepancy of 45degrees. An electron-density map obtained using this phasing algorithm and the measured triplet phases shows good agreement with the known protein structure.
学科主题	Biochemistry & Molecular Biology; Biophysics; Crystallography
类目[WOS]	Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biophysics ; Crystallography
研究领域[WOS]	Biochemistry & Molecular Biology ; Biophysics ; Crystallography
原文出处	SCI
语种	英语
WOS记录号	WOS:000182546900003
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/238534]
专题	高能物理研究所_多学科研究中心
作者单位	中国科学院高能物理研究所
推荐引用方式 GB/T 7714	Shen, Q,Wang J,Wang, J. Recursive direct phasing with reference-beam diffraction[J]. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY,2003,59(5):809-814.
APA	Shen, Q,王俊,&Wang, J.(2003).Recursive direct phasing with reference-beam diffraction.ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY,59(5),809-814.
MLA	Shen, Q,et al."Recursive direct phasing with reference-beam diffraction".ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY 59.5(2003):809-814.