Nanoscale X-Ray Microscopic Imaging of Mammalian Mineralized Tissue

	Nanoscale X-Ray Microscopic Imaging of Mammalian Mineralized Tissue
	Andrews, JC; Almeida, E; van der Meulen, MCH; Alwood, JS; Lee, C; Liu, YJ; Chen, J; Meirer, F; Feser, M; Gelb, J
刊名	MICROSCOPY AND MICROANALYSIS
	2010
卷号	16 期号:3 页码:327-336
关键词	X-ray microscopy imaging bone ultrastructure bone mineral density trabeculae lacuna osteocyte
通讯作者	[Andrews, Joy C. ; Pianetta, Piero] SLAC Natl Accelerator Lab, Stanford Synchrotron Radiat Lightsource, Menlo Pk, CA 94025 USA ; [Almeida, Eduardo] NASA, Ames Res Ctr, Moffett Field, CA 94035 USA ; [van der Meulen, Marjolein C. H.] Cornell Univ, Sibley Sch Mech & Aerosp Engn, Ithaca, NY 14853 USA ; [Alwood, Joshua S.] Stanford Univ, Dept Aeronaut & Astronaut, Stanford, CA 94305 USA ; [Lee, Chialing] San Jose State Univ, Dept Biol Sci, San Jose, CA 95192 USA ; [Liu, Yijin] Inst High Energy Phys, Beijing 100039, Peoples R China ; [Chen, Jie] Univ Sci & Technol China, Natl Synchrotron Radiat Lab, Hefei 230026, Peoples R China ; [Meirer, Florian] Vienna Univ Technol, Inst Atom & Subatom Phys, Vienna, Austria ; [Feser, Michael ; Gelb, Jeff ; Rudati, Juana ; Tkachuk, Andrei ; Yun, Wenbing] Xradia Inc, Concord, CA 94520 USA
英文摘要	A novel hard transmission X-ray microscope (TXM) at the Stanford Synchrotron Radiation Light-source operating from 5 to 15 keV X-ray energy with 14 to 30 mu m(2) field of view has been used for high-resolution (30-40 nm) imaging and density quantification of mineralized tissue. TXM is uniquely suited for imaging of internal cellular structures and networks in mammalian mineralized tissues using relatively thick (50 mu m), untreated samples that preserve tissue micro-and nanostructure. To test this method we performed Zernike phase contrast and absorption contrast imaging of mouse cancellous bone prepared under different conditions of in vivo loading, fixation, and contrast agents. In addition, the three-dimensional structure was examined using tomography. Individual osteocytic lacunae were observed embedded within trabeculae in cancellous bone. Extensive canalicular networks were evident and included processes with diameters near the 30-40 nm instrument resolution that have not been reported previously. Trabecular density was quantified relative to rod-like crystalline apatite, and rod-like trabecular struts were found to have 51-54% of pure crystal density and plate-like areas had 44-53% of crystal density. The nanometer resolution of TXM enables future studies for visualization and quantification of ultrastructural changes in bone tissue resulting from osteoporosis, dental disease, and other pathologies.
学科主题	Materials Science; Microscopy
类目[WOS]	Materials Science, Multidisciplinary ; Microscopy
研究领域[WOS]	Materials Science ; Microscopy
原文出处	SCI
语种	英语
WOS记录号	WOS:000277902600011
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/236996]
专题	高能物理研究所_多学科研究中心
作者单位	中国科学院高能物理研究所
推荐引用方式 GB/T 7714	Andrews, JC,Almeida, E,van der Meulen, MCH,et al. Nanoscale X-Ray Microscopic Imaging of Mammalian Mineralized Tissue[J]. MICROSCOPY AND MICROANALYSIS,2010,16(3):327-336.
APA	Andrews, JC.,Almeida, E.,van der Meulen, MCH.,Alwood, JS.,Lee, C.,...&刘宜晋.(2010).Nanoscale X-Ray Microscopic Imaging of Mammalian Mineralized Tissue.MICROSCOPY AND MICROANALYSIS,16(3),327-336.
MLA	Andrews, JC,et al."Nanoscale X-Ray Microscopic Imaging of Mammalian Mineralized Tissue".MICROSCOPY AND MICROANALYSIS 16.3(2010):327-336.