Nitrate reductase-mediated NO production enhances Cd accumulation in Panax notoginseng roots by affecting root cell wall properties

CORC > 西双版纳热带植物园 > 中国科学院西双版纳热带植物园 > 2012年后新成立研究组

	Nitrate reductase-mediated NO production enhances Cd accumulation in Panax notoginseng roots by affecting root cell wall properties
	Xu, Jin
刊名	JOURNAL OF PLANT PHYSIOLOGY
	2016
卷号	193 期号:x 页码:64-70
关键词	Panax notoginseng Cd accumulation Nitrate reductase Nitric oxide Hemicellulose 1 Pectin
中文摘要	Panax notoginseng (Burk) F.H. Chen is a traditional medicinal herb in China. However, the high capacity of its roots to accumulate cadmium (Cd) poses a potential risk to human health. Although there is some evidence for the involvement of nitric oxide (NO) in mediating Cd toxicity, the origin of Cd-induced NO and its function in plant responses to Cd remain unknown. In this study, we examined NO synthesis and its role in Cd accumulation in P. notoginseng roots. Cd-induced NO production was significantly decreased by application of the nitrate reductase inhibitor tungstate but not the nitric oxide synthase inhibitor L-NAME (NG-methyl-l-arginine acetate), indicating that nitrate reductase is the major contributor to Cd-induced NO production in P. notoginseng roots. Under conditions of Cd stress, sodium nitroprusside (SNP, an NO donor) increased Cd accumulation in root cell walls but decreased Cd translocation to the shoot. In contrast, the NO scavenger cPTIO (2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide) and tungstate both significantly decreased NO-increased Cd retention in root cell walls. The amounts of hemicellulose 1 and pectin, together with pectin methylesterase activity, were increased with the addition of SNP but were decreased by cPTIO and tungstate. Furthermore, increases or decreases in hemicellulose 1 and pectin contents as well as pectin methylesterase activity fit well with the increased or decreased retention of Cd in the cell walls of P. notoginseng roots. The results suggest that nitrate reductase-mediated NO production enhances Cd retention in P. notoginseng roots by modulating the properties of the cell wall.
公开日期	2016-06-06
内容类型	期刊论文
源URL	[http://ir.xtbg.org.cn/handle/353005/9884]
专题	西双版纳热带植物园_2012年后新成立研究组
推荐引用方式 GB/T 7714	Xu, Jin. Nitrate reductase-mediated NO production enhances Cd accumulation in Panax notoginseng roots by affecting root cell wall properties[J]. JOURNAL OF PLANT PHYSIOLOGY,2016,193(x):64-70.
APA	Xu, Jin.(2016).Nitrate reductase-mediated NO production enhances Cd accumulation in Panax notoginseng roots by affecting root cell wall properties.JOURNAL OF PLANT PHYSIOLOGY,193(x),64-70.
MLA	Xu, Jin."Nitrate reductase-mediated NO production enhances Cd accumulation in Panax notoginseng roots by affecting root cell wall properties".JOURNAL OF PLANT PHYSIOLOGY 193.x(2016):64-70.