A fluorescence turn-on detection of copper(II) based on the template-dependent click ligation of oligonucleotides | |
Wang,Fangyuan; Li,Yongxin; Li,Wenying; Chen,Jian; Zhang,Qingfeng; Shahzad,Sohail Anjum; Yu,Cong | |
刊名 | talanta
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2015 | |
卷号 | 132期号:2页码:72-76 |
关键词 | GOLD NANOPARTICLES MOLECULAR BEACONS SELECTIVE DETECTION VISUAL DETECTION ENERGY-TRANSFER GRAPHENE OXIDE CU2+ DETECTION CHEMISTRY IONS PROBES |
通讯作者 | yu,c |
英文摘要 | in this work, a fluorescence turn-on method for copper(ii) detection is reported. a molecular beacon (mb) was designed as a template. cu2+ was reduced to cu+ in the presence of a reductant (ascorbic acid). two short single-stranded oligonucleotides one was labeled with a 5'-alkyne and the other with 3'-azide group, proceeded a template-dependent chemical ligation through the cu(i)-catalyzed azide-alkyne cycloaddition. the newly generated click-ligated long chain oligonucleotide, which was complementary to the mb, opened the mb hairpin structure and resulted in a turn on fluorescence. the increase in fluorescence intensity is directly proportional to the amount of cu2+ added to the assay solution. the present assay is quite sensitive and allows the detection of 2 nm cu2+. the described assay also exhibits high selectivity over other metal ions. (c) 2014 elsevier b.v. all rights reserved. |
收录类别 | SCI |
语种 | 英语 |
公开日期 | 2016-05-31 |
内容类型 | 期刊论文 |
源URL | [http://ir.ciac.jl.cn/handle/322003/67901] ![]() |
专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
推荐引用方式 GB/T 7714 | Wang,Fangyuan,Li,Yongxin,Li,Wenying,et al. A fluorescence turn-on detection of copper(II) based on the template-dependent click ligation of oligonucleotides[J]. talanta,2015,132(2):72-76. |
APA | Wang,Fangyuan.,Li,Yongxin.,Li,Wenying.,Chen,Jian.,Zhang,Qingfeng.,...&Yu,Cong.(2015).A fluorescence turn-on detection of copper(II) based on the template-dependent click ligation of oligonucleotides.talanta,132(2),72-76. |
MLA | Wang,Fangyuan,et al."A fluorescence turn-on detection of copper(II) based on the template-dependent click ligation of oligonucleotides".talanta 132.2(2015):72-76. |
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