A fluorescence turn-on detection of copper(II) based on the template-dependent click ligation of oligonucleotides

	A fluorescence turn-on detection of copper(II) based on the template-dependent click ligation of oligonucleotides
	Wang,Fangyuan; Li,Yongxin; Li,Wenying; Chen,Jian; Zhang,Qingfeng; Shahzad,Sohail Anjum; Yu,Cong
刊名	talanta
	2015
卷号	132 期号:2 页码:72-76
关键词	GOLD NANOPARTICLES MOLECULAR BEACONS SELECTIVE DETECTION VISUAL DETECTION ENERGY-TRANSFER GRAPHENE OXIDE CU2+ DETECTION CHEMISTRY IONS PROBES
通讯作者	yu,c
英文摘要	in this work, a fluorescence turn-on method for copper(ii) detection is reported. a molecular beacon (mb) was designed as a template. cu2+ was reduced to cu+ in the presence of a reductant (ascorbic acid). two short single-stranded oligonucleotides one was labeled with a 5'-alkyne and the other with 3'-azide group, proceeded a template-dependent chemical ligation through the cu(i)-catalyzed azide-alkyne cycloaddition. the newly generated click-ligated long chain oligonucleotide, which was complementary to the mb, opened the mb hairpin structure and resulted in a turn on fluorescence. the increase in fluorescence intensity is directly proportional to the amount of cu2+ added to the assay solution. the present assay is quite sensitive and allows the detection of 2 nm cu2+. the described assay also exhibits high selectivity over other metal ions. (c) 2014 elsevier b.v. all rights reserved.
收录类别	SCI
语种	英语
公开日期	2016-05-31
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/67901]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Wang,Fangyuan,Li,Yongxin,Li,Wenying,et al. A fluorescence turn-on detection of copper(II) based on the template-dependent click ligation of oligonucleotides[J]. talanta,2015,132(2):72-76.
APA	Wang,Fangyuan.,Li,Yongxin.,Li,Wenying.,Chen,Jian.,Zhang,Qingfeng.,...&Yu,Cong.(2015).A fluorescence turn-on detection of copper(II) based on the template-dependent click ligation of oligonucleotides.talanta,132(2),72-76.
MLA	Wang,Fangyuan,et al."A fluorescence turn-on detection of copper(II) based on the template-dependent click ligation of oligonucleotides".talanta 132.2(2015):72-76.